侧脑室注射NMDA受体激动剂后精神分裂症小鼠海马齿状回内NR1和BrdU的改变  

Change of NR1 and BrdU in the hippocampal dentate gyrus in schizophrenia mice by intracerebroventricular injection of NMDA receptor agonist

作　　者：吕玥[1] 肖培伦[1] 马全瑞[1] 马江波[1] 周惠惠[1] 张潇剑[1] 刘娟[1,2] 

机构地区：[1]宁夏医科大学人体解剖与组织胚胎学系,银川750004 [2]宁夏医科大学宁夏颅脑疾病重点实验室,银川750004

出　　处：《神经解剖学杂志》2015年第1期54-58,共5页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(81160169);教育部春晖计划(Z2011042)

摘　　要：目的:探讨精神分裂症(schizophrenia,SP)小鼠侧脑室注射N-甲基-D-天门冬氨酸(NMDA)受体激动剂后,海马齿状回(dentate gyrus,DG)颗粒细胞层NR1和5-溴脱氧尿嘧啶核苷(BrdU)阳性细胞数量的改变。方法:选择健康6周龄雄性C57小鼠,地卓西平马来酸盐(MK-801)慢性给药制备精神分裂症动物模型,实验分为NMDA组、生理盐水组、SP组和空白对照组共四组,NMDA组侧脑室注射NMDA受体激动剂。BrdU标记后行免疫荧光染色,激光共聚焦显微镜下观察和计数DG颗粒细胞层NR1和BrdU阳性细胞的表达和数量变化。结果:(1)NR1阳性细胞数在NMDA组、空白对照组、SP组和生理盐水组分别为16.1±2.08,14.5±2.17,19.4±4.65,19.5±4.33,经比较,NMDA组和空白对照组NR1阳性细胞的数量明显少于SP组(P<0.05),但NMDA组与空白对照组相比无明显差异(P>0.05),SP组与生理盐水组相比也无明显差异(P>0.05)。(2)BrdU阳性细胞数在NMDA组、空白对照组、SP组和生理盐水组分别为5.2±2.53、5.3±0.82、3.4±1.58、3.5±1.35,经比较,NMDA组和空白对照组分别多于SP组(P<0.05),和生理盐水组(P<0.05);但NMDA组较空白对照组无明显差异(P>0.05),SP组较生理盐水组亦无明显差异(P>0.05)。结论:SP小鼠侧脑室注射NMDA受体激动剂后,可引起DG颗粒层NR1阳性细胞数减少和BrdU阳性神经细胞数的增多。Objective： To investigate the expression changes of NR1 and BrdU in the hippocampal dentate gyrus （DG） of schizophrenia （SP） model mice that subject to intracerebroventricular injection of N-metthy-D-aspartic acid （NMDA） receptor agonist. Methods： Healthy 6-week-old male mice were used to establish the SP model by intraperitoneal admin- istration of MK-801. Grouping： intracerebroventricular injection of NMDA receptor agonist group （NMDA group） ; intrac- erebroventricular injection of saline group （ saline group） ; schizophrenia group （ SP group） and no drug group （ control group）. After 5-Bromo-2-deoxyUridine （BrdU） labeling, immunofluoresence histochemical staining was used to observe and count the expression and number of NR1 and BrdU in the DG granular layer under confocal laser scaning microscope.Results ： （ 1 ） The number of NR1 positive cells in NMDA group, blank group, SP group and saline group are respectively 16.1 ± 2.08, 14.5 ± 2.17, 19.4 ±4.65, 19.5 ± 4.33. After being compared, the number of NR1 positive ceils both in NMDA group and blank group were respectively less than that in SP group. But there was no difference between NMDA group and blank group （ P 〉 0.05 ） , also no difference between SP group and saline group （ P 〉 0.05 ）. （ 2 ） The number of BrdU positive cells in NMDA group, blank group, SP group and saline group were respectively 5.2 ± 2.53, 5.3 ± 0.82, 3.4 ± 1.58, 3.5 ±1.35. After being compared, the number of NR1 positive cells both in NMDA group and blank group were respectively more than that in SP group, and in saline group. But there was no difference between NMDA group and blank group （ P 〉 0. 05 ） , also no difference between SP group and saline group （ P 〉 0. 05 ）. Conclusions ： After intracerebroventricular injection of NMDA receptor agonist in SP model mice, the number of NR1 positive cells in DG granular layer decreased, while the number of BrdU positive cells in DG granular layer increased.

关 键 词：精神分裂症 海马 NMDA NR1 BRDU 小鼠 

分 类 号：R749.3[医药卫生—神经病学与精神病学]