HSL基因重复多态与有氧耐力的关联性  

Studies on association of HSL repeat polymorphism and aerobic endurance

作　　者：夏小慧[1] 夏惠芸 郭洁[1] 刘康超[1] 胡扬[3] 

机构地区：[1]兰州城市学院 [2]国营长风机器厂职工医院,甘肃兰州730070 [3]北京体育大学,北京100084

出　　处：《中国应用生理学杂志》2015年第1期93-96,共4页Chinese Journal of Applied Physiology

基　　金：甘肃省自然科学基金(1208RJZA165);国家科技攻关计划项目(2003BA904B04)

摘　　要：目的:探讨激素敏感性脂肪酶(HSL)基因重复多态与有氧耐力之间的关联性。方法:采用PCR结合荧光标记复合STR-genescan方法,对123名中国北方汉族优秀长跑运动员与127名中国北方汉族普通大学生HSL基因第6内含子区(CA)n重复多态基因分型进行检测,对重复多态按分割点进行分组,划分为长、短链两个等位基因,卡方检验比较运动员组和对照组间等位基因频率和基因型频率差别。结果:HSL基因(CA)n重复多态共有9种不同重复次数的等位基因,组成25种不同基因型。卡方检验结果显示,当以4重复次数为分割点划分短链和长链等位基因进行比较时,基因型的分布在5/10 km组与对照组相比,有显著性差异(P<0.05)。其余各组间进行比较,均无显著性差异。结论:HSL基因第6内含子(CA)n重复多态,当以4重复次数为分割点划分短链和长链等位基因进行比较时,LL基因型与有氧耐力相关联,可能是5/10 km运动员的分子标记。Objective： To investigate the relationship between hormone sensitive lipase（ HSL） gene polymorphism and aerobic endurance. Methods： The（ CA） n repeats polymorphism genotypes in HSL intro 6 of 123 outstanding long distance runners and 127 controls of Han nationality in northern China were analyzed by PCR and Fluorescence labeled STR-genescan. Repeat polymorphisms were grouped according to segmentation point and alleles were divided into long or short chains. Chi-square test was used to analyze the frequency difference of allelic and genotypic between athlete and control groups. Results：（ CA） n repeats polymorphism in HSL gene was total of 9 different repeat number of alleles,which composed of 25 different genotypes. The chi-square test result showed that when compared short and long chain alleles split by 4,there was a significant difference（ P 〈0. 05） of genotype distribution in 5 /10 km group compared with control. Compared the rest groups with control,there was no significant difference. Conclusion： Compared short and long chain alleles split by 4,the LL genotype of（ CA） n of HSL was associated with aerobic endurance and it might be a molecular marker of elite 5 /10 km long distance runners.

关 键 词：HSL 基因多态性 有氧耐力 分子标记 

分 类 号：R363.1[医药卫生—病理学]