高效液相色谱-串联质谱法测定豆芽中8种药物残留  

Simultaneous Determination of Eight Kinds of Drug Residues in Sprouts by High Performance Liquid Chromatography- Tandem Mass Spectrometry

作　　者：张亚莲[1] 柳菡[2] 王岁楼[1] 丁涛[2] 徐牛生 余可垚[2] 沈伟健[2] 赵增运[2] 刘芸[2] 吴斌[2] 张睿[2] 沈崇钰[2] 

机构地区：[1]中国药科大学药学院,江苏南京210009 [2]江苏出入境检验检疫局,江苏南京210001 [3]赛默飞世尔科技色谱质谱部,上海201206

出　　处：《分析测试学报》2015年第2期164-170,共7页Journal of Instrumental Analysis

基　　金：国家科技支撑计划课题(2014BAK19B02;2012BAK08B01;2015KJ29)

摘　　要：建立了豆芽中8种药物（4-氯苯氧乙酸、吲哚乙酸、吲哚丁酸、1-萘乙酸、6-苄基腺嘌呤、2,4-二氯苯氧乙酸、赤霉素和多菌灵）残留的高效液相色谱-串联质谱（LC-MS/MS）检测方法。豆芽样品经0.1%冰醋酸-乙腈溶液提取、浓缩,分散固相萃取剂净化后,用液相色谱-串联质谱测定,外标法定量。8种药物在5-100μg/L范围内呈良好的线性关系（r^2〉0.99）,定量下限为5μg/kg。在5,10,20μg/kg 3个加标水平下,8种药物的回收率为71.6%-87.9%,相对标准偏差不大于14.6%。方法准确、简单、快速,可用于豆芽中8种药物的同时测定。A high performance liquid chromatography- tandem mass spectrometric（ LC- MS / MS）method was developed for the determination of eight kinds of drugs（ 4-chlorophenoxyacetic acid,indole-3-acetic acid,2,4-dichlorophenoxyacetic acid, indole-3-butyric acid,1-naphthlcetic acid,gibberellin acid,6-benzylaminopurine and carbendazim） in sprouts. The samples were extracted with acetonitrile containing 0. 1% acetic acid, concentrated and cleaned up with dispersive solid phase extraction powder. The extract was analyzed by LC- MS / MS. The linear ranges of eight compounds ranged from 5 μg / L to 100 μg / L with correlation coefficients（ r^2） more than 0. 99. The limits of quantitation of eight compounds were 5 μg / kg. At three spiked concentration levels of 5,10,20μg /kg,the recoveries of eight compounds ranged from 71. 6% to 87. 9% with relative standard deviations（ RSDs） not more than 14. 6%. The method was accurate,simple and quick,and was suitable for the simultaneous determination of eight compound residues in sprouts.

关 键 词：高效液相色谱-串联质谱法(LC-MS/MS) 豆芽 药物残留 

分 类 号：O657.63[理学—分析化学] TQ460.72[理学—化学]