两种反胶束固定化酶的催化性质研究  

Study on enzymatic characterizations of the immobilized enzymes in reversed micelle

作　　者：朱苗苗[1] 柳畅先[1] 

机构地区：[1]中南民族大学化学与材料科学学院 分析化学国家民委重点实验室,湖北武汉430074

出　　处：《化学研究与应用》2015年第2期168-171,共4页Chemical Research and Application

基　　金：中南民族大学自然科学基金项目(XTZ09005)资助

摘　　要：探讨十六烷基三甲基氯化铵(CATC)-戊醇-辛烷反胶束体系固定化乳酸脱氢酶(LDH)和醇脱氢酶(ADH)的催化作用。试验了含水量、表面活性剂以及助溶剂浓度对于酶固载量的影响。对游离酶和固定化酶催化性质研究表明:LDH酶促反应的适宜p H值分别为9.2和9.0,适宜温度为51℃和29℃,米氏常数15mmol·L-1和2mmol·L-1;ADH酶促反应的适宜p H值为8.8和8.2,适宜温度为33℃和39℃,米氏常数4mmol·L-1和17mmol·L-1。应用反胶束固定化酶测定了试样中微量组分。The enzymatic kinetics of immobilized lactate dehydrogenase（ LDH） and alcohol dehydrogenase（ ADH） with cetyl trimthyl ammonium chloride（ CTAC）-pentanol-octane reversed micelle system were investigated. The effect of the level of water,the concentration of surfactant and cosolvent on the immobilization of LDH and ADH were assayed. The enzymatic characterizations of free and immobilized enzymes were also studied. In the case of LDH,the optimum p H of enzymatic reaction were 9. 2 and 9. 0 for the free and immobilized ones,respectively the optimum temperature were 51℃ and 29℃,respectively the michaelis constant were 15mmol·L^-1and 2mmol·L^-1,respectively. With regard to ADH,the optimum p H of enzymatic reaction were 8. 8 and 8. 2 for the free and immobilized ones,respectively the optimum temperature were 33℃ and 39℃,respectively the michaelis constant were 4mmol·L^-1and 17mmol·L^-1,respectively. The immobilized enzymes could be used to determine trace component in samples.

关 键 词：反胶束 固定化 乳酸脱氢酶 醇脱氢酶 酶催化作用 

分 类 号：O629.8[理学—有机化学]