聚合度11-15大环糊精的分离  

Isolation of cycloamylose with degree of polymerization between 11 and 15

作　　者：杨成[1] 刘书磊[1] 王水兴[2] 

机构地区：[1]南昌大学环境与化学工程学院,江西南昌330031 [2]南昌大学中德联合研究院,江西南昌330047

出　　处：《现代化工》2014年第12期54-57,共4页Modern Chemical Industry

基　　金：江西省教育厅资助项目(GJJ11290)

摘　　要：利用α-糖基转移酶催化玉米淀粉制备了纯度约为46％的大环糊精.同时利用Bio-Gel P-4（Fine,45～ 90 μm）柱层析对大环糊精进行初步分离,通过硅胶（200～ 300目）柱层析进一步分离,用薄层色谱法和高效液相法对分离液进行鉴别和纯度分析,分别得到纯度为92.4％、90.2％、86.7％、84.5％、85.3％的11-15单一聚合度大环糊精.Cycloamylose with the purity about 46% is prepared by catalyzing the corn starch using a- glyeosyhransferase. Preliminary separation of obtained eycloamylose is performed by Bio-Gel P-4 （Fine,45-90） column chromatography. Silica gel is used to further purify the cycloamylose. TLC and HPLC are used to identify the separation process and analyze the purity of the resulting cycloamylose. It shows that the purity of cycloamylose with single degree of polymerization between II and 15 is 92.4% ,90. 2% ,86. 7% ,84. 5% and 85.3% ,respectively.

关 键 词：大环糊精 分离 柱层析 

分 类 号：TS236.9[轻工技术与工程—粮食、油脂及植物蛋白工程]