松木层孔菌杂多糖中的3-O-甲基-半乳糖的分析鉴定  

Identification of 3-O-methyl-galactose in heteropolysaccharide isolated from the fruiting body of Phellinus pini

作　　者：杨开[1,2] 张佳妍 邢辰[1] 金月忠 张安强[1,3] 胡江宁 孙培龙[1] 

机构地区：[1]浙江工业大学食品系,杭州310014 [2]浙江中药与天然药物研究院,杭州310052 [3]杭州百山祖生物科技有限公司,杭州310051

出　　处：《药物分析杂志》2015年第2期235-240,共6页Chinese Journal of Pharmaceutical Analysis

基　　金：浙江省自然科学基金资助项目(LY13C200011);国家科技支撑计划资助项目(No.2013BAD16B07)

摘　　要：目的:分析鉴定松木层孔菌子实体杂多糖中的未知单糖组分。方法:松木层孔菌子实体经提取分离纯化得到水溶性均一多糖组分PP80W,HPLC法测定其均一性和分子量。PP80W经水解、还原和乙酰化后采用GC法对照测定其单糖组分,并对其中未知的1个单糖组分采用GC-MS、DEPT-135和2D NMR(1H-1H COSY、TOCSY、HMQC、HMBC和NOESY谱)进一步分析鉴定。结果:PP80W相对分子质量为2.99×103,GC分析发现PP80W由鼠李糖、甘露糖、葡萄糖、半乳糖和1种未知单糖构成,GC-MS和核磁共振分析鉴定该未知单糖为3-O-甲基-半乳糖。结论:PP80W为含有3-O-甲基-半乳糖结构的杂多糖组分。Objective： To identify an unknown sugar component in polysaccharide PP80 W purified from the fruiting body of Phellinus pini. Methods： A water-soluble and mono-disperse polysaccharide PP80 W was obtained from the fruiting body of Phellinus pini by hot water extraction,separation and purification. The homogeneity and molecular weight of PP80 W were analyzed by HPLC. PP80 W was hydrolyzed with TFA,reduced with Na BH4 and acetylated with acetic anhydride. Then the monosaccharide composition of PP80 W was analyzed by GC. GC-MS,DEPT-135 and 2D NMR（^1H-^1H COSY,TOCSY,HMQC,HMBC and NOESY spectra） were further used to identify the unknown sugar component. Results： PP80 W,with a relative molecular mass of 2. 99 × 10^3,was determined to be composed of rhamnose,mannose,glucose,galactose and an unknown monosaccharide by GC analysis. The unknown monosaccharide was identified as 3-O-methyl-galactose by GC-MS and NMR analysis. Conclusion： PP80 W was a heteropolysaccharide contain 3-O-methyl-galactose.

关 键 词：药用菌 松木层孔菌(松针层孔菌 松白腐菌) 多糖分析鉴定 3-O-甲基-半乳糖 气相色谱 气质联用 核磁共振 

分 类 号：R917[医药卫生—药物分析学]