贵州省水族和仡佬族永生细胞系建立  

Establishment of immortal cell lines from Shui and Gelao ethnic populations in Guizhou province

作　　者：黎明[1] 张广超[2] 孙良先[1] 唐莉娜[1] 杨敬源[2] 褚嘉祐[3] 杨昭庆[3] 

机构地区：[1]贵州省疾病预防控制中心,贵阳550004 [2]贵阳医学院 [3]中国医学科学院北京协和医学院医学生物学研究所

出　　处：《中国公共卫生》2015年第3期306-308,共3页Chinese Journal of Public Health

基　　金：国家高技术研究发展计划(863)(2012AA021802);贵州省科学技术基金[(2012)2211号]

摘　　要：目的建立贵州省水族和仡佬族少数民族永生细胞系,为生命科学研究提供技术支持。方法采用EB病毒(EBV)感染和环孢霉素A(Cy A)处理方法,对来自贵州省三都县的144名水族和来自务川县的124名仡佬族人群外周静脉血B淋巴细胞进行转化,并对转化的细胞进行细胞形态学观察,用直接计数法绘制转化细胞的生长曲线并计算细胞倍增时间,对转化的细胞进行传代、冻存和复苏实验,采用培养法进行支原体污染检测。结果成功建立了138个水族和115个仡佬族人群的永生化B淋巴细胞株,转化率分别为95.8%和92.7%;转化的B淋巴细胞呈现典型的细胞变形和克隆化生长特点,能持续传代、冻存复苏后生长良好,无支原体污染。结论贵州省水族和仡佬族人群B淋巴细胞被成功转化为永生化细胞系。Objective To establish an immortal cell line from Shui and Gelao ethnic populations in Guizhou province and to provide a permanent research material for life science research. Methods Totally 144 peripheral venous blood samples from Shui ethnic people in Sandu county and 124 from Gelao in Wuchuan county in Guizhou province were collected. B lymphocytes isolated from the blood samples were transformed using Epstein-Barr（ EB） virus infection and cyclsoporine A（ Cy A） treatment. The morphology of the transformed cells was observed and direct counting method was used to make cell growth curve and to calculate cell doubling time of the transformed cells. Passage,frozen storage,and recovery of the transformed cells were performed and culture was conducted to detect mycoplasma contamination.Results Totally 138 immoral B lymphocyte cell lines from Shui ethnic group and 115 from Gelao ethnic group were successfully established with the conversion rates of 95. 8% and 92. 7%,respectively. The transfored B lymphocytes present typical cell deformation,cloning growth characteristics,and normal growth after recovery,without mycoplasma contimination. Conclusion Peripheral B lymphocytes from Shui and Gelao ethnic populations were transformed into immortal cell lines successfully for further researches.

关 键 词：EB病毒(EBV) 永生细胞 B淋巴细胞 水族 仡佬族 

分 类 号：R181.24[医药卫生—流行病学]