孕期及哺乳期不同含量n-3多不饱和脂肪酸对子代成年期小鼠海马神经细胞凋亡相关因子的影响  

Dietary ratios of N-6 / n-3 polyunsaturated fatty acids during maternal pregnancy and lactation affect apoptotic proteins of hippocampus in adult offspring of mice

作　　者：高亚兵[1] 付慧聪 樊超男[2] 董华[2] 路媛媛[2] 夏露露[2] 丁绪 董霁[1] 彭瑞云[1] 齐可民[2] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850 [2]首都医科大学附属北京儿童医院,北京市儿科研究所,儿科学国家重点学科,教育部儿科重大疾病研究重点实验室,北京100045

出　　处：《中国体视学与图像分析》2014年第4期380-386,共7页Chinese Journal of Stereology and Image Analysis

基　　金：国家自然科学基金资助项目(81100630);北京市科技新星项目(XX2012047)

摘　　要：目的观察母孕期及哺乳期不同含量n-3多不饱和脂肪酸（PUFAs）饲料对成年期仔鼠脑神经细胞凋亡的影响。方法使用6～8W龄清洁级C57BL／6J雌性小鼠，随机分为5组，每组10只，分别给予n-3 PUFAs缺乏和3种不同比例n-6／n-3PUFAs（n-6／n-3PUFAs比值分别为15：1、5：1、1：1）饲料及1种高含量鱼油n一3PUFAs饲料（n-6／n-3PUFAs比值为1：5）喂养。小鼠12～14W龄时雌雄合笼交配繁殖，仔鼠断乳后继续行母鼠相同饲料喂养，选取生后3m成年仔鼠用于实验。取脑进行组织固定，采用免疫组织化学技术对脑组织海马区Bcl-2和BaX表达进行定量分析。结果与n-3PUFAs缺乏组相比，n-3PUFAs饲料喂养组，尤其是13—6／n-3PUFAs比值（5：1）和（1：1）组小鼠海马CA3区神经细胞抗凋亡蛋白Bcl-2表达明显增加（P〈0．05），而致凋亡蛋白Bax表达则显著降低（P〈0．05）。但高含量鱼油n一3PUFAs喂养组（即n-6／n-3PUFAs1：5组）与n一3PUFAs缺乏组相比未表现出显著性差异。结论孕期及哺乳期添加13—3PUFAs，尤其是n～6／n-3PUFAs比值在5～1：1之间时有助于减少成年仔鼠脑组织神经细胞凋亡的发生，而过高含量n-3PUFAs摄入则未对脑凋亡发生起到积极作用。Objective To investigate the effects of dietary n-6/n-3 PUFA ratios during pregnancy lactation on hippocampal apoptosis in the brain of adult offspring. Methods Forty 6-8 week-old C57BL/ 6J female mice were randomly divided into 5 groups. Mice were fed with five types of diet, n-3 PUFAs de- ficient diet, three n-3 PUFAs diets with different ratios of n-6/n-3 PUFAs （15：1 , 5： 1, and 1：1 ） and a high fish oil diets with n-6/n-3 in ratio of 1 ： 5. Followed by 6 weeks feeding, the mice weremated for breeding pups. The mice after 21 d received the same diets as their mothers had received. At postnatal Results As compared with n-3 PUFA deficient diet, n-3 PUFA containing diets particularly with n-6/n- 3 PUFA ratios at 5：1 and 1 ： 1, significantly increased the expression of Bcl-2 protein and decreased the expression of Bax protein in hippocampus CA3 of the offspring mice. However, these parameters showed no significant differences between the high fish oil diet group and n-3 PUFA deficient diet group. Conclusions The results of this study suggest that the higher intake amount of n-3 PUFAs with a low ra- tio of n-6/n-3 PUFAs at about 5-1： 1, supplied during both maternal pregnancy and lactation, may be more beneficial for offspring brain apoptosis, and over intake of n-3 PUFAs may exert a negative influence on the offspring brain.

关 键 词：N-3多不饱和脂肪酸 小鼠 海马 凋亡 图像分析 

分 类 号：R151[医药卫生—营养与食品卫生学]