长柄扁桃仁多酚提取工艺及抗氧化活性研究  

Extraction of Polyphenols fromAmygdalus pedunculata Seed and the Antioxidant Capacity

作　　者：苏锐[1] 樊金拴[1] 郑涛[1] 赵韵美[1] 马菁[1] 高智辉[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨陵712100

出　　处：《西北林学院学报》2015年第2期206-212,共7页Journal of Northwest Forestry University

基　　金：陕西省经济植物资源利用重点实验室专项经费;国家林业推广项目"黄土高原核桃优质丰产栽培配套技术示范推广"(2011-23);国家林业局林业公益行业科研专项资助项目"长柄扁桃优良品种选育及丰产栽培技术研究"(201104074)

摘　　要：运用单因素及正交试验研究超声辅助提取长柄扁桃仁中多酚的最佳工艺,采用DPPH法、ABTS法、FRAP法对比5个不同种源地样品的抗氧化活性。结果表明,长柄扁桃仁中超声辅助提取的最佳工艺为:乙醇体积分数20%,料液比1∶20,提取温度40℃,超声时间60min,提取率为2.076mg·g-1。对提取效果影响大小的因素依次为乙醇体积分数>料液比>提取温度>时间。采自陕北地区的种仁中提取的多酚抗氧化活性相对其他2个种源地较强。最佳工艺可有效提高多酚提取量;在适宜环境中生长的长柄扁桃中的仁有效成分含量较高,抗氧化活性较强。Single factor and orthogonal experiments were adopted to examine the optimal procedure of ultrasound aided extraction of phenols from the kernel of Amygdalus pedunculata.The antioxidant activity of the extracts was measured by DPPH free radical method,ABTS and FRAP methods.The optimal conditions were obtained as solvent：20% ethanol,the ratio of material being extracted to solvent：1∶20,temperature：40℃,time of extraction aided by ultrasound：60min,under these conditions,the yield of extract was 2.08mg/g.The impacts of the factors influencing the extraction were ethanol concentration timeratio of material to solventtemperature.The antioxidant activities of the extracts from the materials collected from northern Shaanxi were higher than those from other locations,indicating that suitable growing conditions were favorable for the accumulation of phenols.

关 键 词：长柄扁桃仁 超声提取工艺 多酚 抗氧化活性 

分 类 号：S713[农业科学—林学]