菊粉酶产生菌的筛选、鉴定及发酵条件优化  

Isolation and identification of a inulinase-producing strain and optimization of its fermentation condition

作　　者：朱宏阳[1] 冯珊[1] 王金海[1] 李泳宁[1] 林伟铃[1] 

机构地区：[1]福建卫生职业技术学院,福州350101

出　　处：《海峡药学》2015年第1期214-218,共5页Strait Pharmaceutical Journal

基　　金：福建省自然科学青年基金项目(2012J05015);福建省教育厅A类科技项目(JA12430)

摘　　要：菊粉酶因其能水解菊粉中β-2,1-D果聚糖果糖苷键而广泛用于高果糖浆、低聚果糖和酒精的生产,在药品和食品工业生产中具有重要的应用价值和良好的应用前景。本文从菊芋根际土壤中筛选分离获得28株菊粉酶产生菌株,经进一步复筛获得一株产菊粉酶能力较高的菌株ni-3,在对其常规形态学及生理生化特性鉴定的基础上,对部分长度的16S r DNA同源性进行了分析,发现ni-3菌株与中孢短芽孢杆菌相似度达99.5%,命名为Brevibacillus centrosporus ZF-9。本文还对其发酵产酶培养基进行单因素试验及响应面优化,确定了ZF-9最佳产酶条件为:菊粉45g·L-1,酵母膏11 g·L-1,Na2HPO420 g·L-1,在该培养基下,菊粉酶活可达6.41 U·m L-1。The inulinases are classified among the hydrolases and target on the β-（ 2 →1）-linkage of inulin and can be used in producing high fructose corn syrup,fructo-oligosaccharides and alcohol,and it has significant potential for the application in food industry. 28 strains capable of producing inulinase were isolated from rhizosphere soil of Jerusalem artichoke（ Helianthus tuberosus L.）. Of all,a bacterial strain ni-3 was isolated through further screening. Based on morphology,physiological and biochemical characteristics,ni-3 was identified by 16 S r DNA sequences and systematic analysis. The results indicated that 16 S r DNA sequences of this strain had 99. 5% similarity with the partial sequence of Brevibacillus centrosporus,suggesting that the strain is a subspecies of B. centrosporus,and denominated as B. centrosporus ZF-9. The optimum conditions of fermentation were investigated through single-factor and response surface method. The results showed that the best conditions for inulinase were shown as follow： inulin 45g·L^- 1,yeast extract 11 g·L^- 1,Na2HPO4 20 g·L^- 1. Under these conditions,inulinase activity of B. centrosporus ZF-9 reached to 6. 41 U·m L^- 1.

关 键 词：菊粉 菊粉酶 Brevibacillus centrosporus 筛选鉴定 条件优化 

分 类 号：TQ460.4[化学工程—制药化工]