真菌诱导子对纳他霉素生物合成的影响  

Infl uence of fungal elicitors on biosynthesis of natamycin

作　　者：王亚洲[1] 古绍彬[1] 刘胜男[1] 王大红[1] 张永柱[1] 

机构地区：[1]河南科技大学食品与生物工程学院,洛阳471023

出　　处：《中国食品添加剂》2015年第2期63-68,共6页China Food Additives

基　　金：国家青年科学基金(31401672);国家自然科学基金(31000017);河南省重点科技攻关项目(122102110034);河南科技大学科研基金(2007QN002)资助

摘　　要：研究黑曲霉、产黄青霉、米曲霉和酿酒酵母四种真菌诱导子对生物防腐剂纳他霉素生物合成的影响,获得具有促进作用的真菌诱导子,并对诱导子的主要作用成分和诱导机理进行初步分析。结果显示:产黄青霉代谢产物诱导子的促进作用最强,使纳他霉素产量达到1.856g/L,较原始产量0.835g/L提高了122.3%;通过优化试验得到最佳诱导条件:产黄青霉诱导子的最优培养时间为2d;在纳塔尔链霉菌发酵24h时加入,发酵至120h,纳他霉素的产量达到2.769g/L,较对照组提高了231.6%;通过有机溶剂萃取分离试验和发酵过程相关参数的检测,确证了真菌代谢产物中起主要诱导活性的是非蛋白、非多糖类的小分子物质,并且其极性与丁醇相近。the influence of the four fungal elicitors of Aspergillus niger, Penicillium chrysogenum, Aspengillus oryzae and Saccharomyces cerevisiae on biosynthesis of natamycin was studied. The screening was to find out acquisition fungal elicitor that induces natamycin production, and major role in the composition and mechanism of induction of elicitor. Results showed that the metabolites elicitor from Penicillium chrysogenum have the strongest acceleration, the production of natamycin was 1.856g/L, which was 122.3% higher than that of original production; When fungal elicitor was added after 24h natamycin fermentation and incubated for 120h, the observed average yield of natamycin was 2.769g/L, which was 231.6% higher than the eontrol; After separation from the organic solvent, the detection of the parameters in the fermentation processing, it confirmed that the main induction activity of fungal elicitors was a small molecular substance with a similar polarity of butanol.

关 键 词：真菌诱导子 纳他霉素 产黄青霉 诱导机理 

分 类 号：TS202.3[轻工技术与工程—食品科学]