新疆小麦高分子量麦谷蛋白亚基组成及主效亚基分析  

Analysis of High-molecular-weight Glutenin Subunit Composition and Main Subunits of Xinjiang Wheat

作　　者：张钰[1] 杨坤[1] 曾卫军[1] 范玲 祝长青[1] 

机构地区：[1]新疆师范大学,新疆乌鲁木齐830054 [2]新疆农业科学院,新疆乌鲁木齐830091

出　　处：《核农学报》2015年第2期229-234,共6页Journal of Nuclear Agricultural Sciences

基　　金：中科院西部之光联合学者项目;新疆维吾尔自治区重点实验室开放项目

摘　　要：为探索新疆强筋小麦的主效亚基,本文采用SDS-PAGE方法对新疆主栽的21种中筋小麦和17种强筋小麦的高分子量麦谷蛋白亚基进行了分析。结果表明:供试材料中共出现12种类型的亚基和12种亚基组合。中筋小麦Glu-A1位点亚基主要为N,Glu-B1位点亚基主要为7+8,Glu-D1位点亚基主要为2+12。强筋小麦Glu-A1位点亚基主要为1和N,Glu-B1位点亚基主要为7+8和7+9,Glu-D1位点亚基主要为5+10。聚类分析结果显示:在相似系数为0.43时可将供试小麦分为2类:第1类包括28个品种,均含有2+12亚基,其中既有强筋小麦也有中筋小麦;第2类包括12个品种,均含有5+10亚基,全部为强筋小麦。5+10亚基对小麦品质贡献明显大于其他亚基,是新疆强筋小麦的主效亚基。本研究可为提高新疆小麦面筋强度的分子育种提供理论依据。In order to explore the main subunits of Xinjiang strong-gluten wheat, the composition of high-molecular- weight glutenin subunits of 21 medium-gluten and 17 strong-gluten wheat eultivars were analyzed by SDS-PAGE. The results showed that there are 12 kinds of subunits and 12 subunit combinations among the experimental materials. Among medium-gluten wheats, major types in locus Glu-A1, Glu-B1 and Glu-D1 are N, 7 ＋ 8 and 2 ＋ 12, while the major types of Glu-A1, Glu-B1 and Glu-D1 in strong-gluten wheats are 1 and N, 7 ＋ 8 and 7 ＋ 9, 5 ＋ 10, respectively. Cluster analysis results showed that the tested materials could be divided into two major groups at the similarity coefficient of 0.43. Group 1 includes 28 varieties, they all have subunits 2 ＋ 12, including strong-gluten vs medium-gluten wheat cultivars. Group 2 includes 12 ones and they are strong-gluten with subunits 5 ＋ 10. The subunits of 5 ＋ I0 contributes more to wheat quality than any other subunits so that it is the main subunit of Xinjiang strong-gluten wheat that providing some theoretical basis for molecular breeding in the improvement of bread-making quality of wheat in Xinjiang zone.

关 键 词：小麦 高分子量麦谷蛋白亚基(HMW-GS) SDS-PAGE 主效亚基 

分 类 号：S512.1[农业科学—作物学]