气相色谱-串联质谱法测定食用植物油中β-谷甾醇含量  

Determination of β-sitosterol in edible vegetable oil using gas chromatography-tandem mass spectrometry

作　　者：钟冬莲[1] 莫润宏[1] 沈丹玉[1] 刘毅华[1] 丁明[1] 汤富彬[1] 

机构地区：[1]中国林业科学研究院亚热带林业研究所,国家林业局经济林产品质量检验检测中心(杭州),浙江富阳311400

出　　处：《中国油脂》2015年第2期95-97,共3页China Oils and Fats

摘　　要：建立了气相色谱-串联质谱法（GC-MS/MS）测定食用植物油中β-谷甾醇含量的分析方法。样品经2 mol/L氢氧化钾-乙醇溶液皂化、石油醚萃取后,采用GC-MS/MS在多反应监测（MRM）模式下对β-谷甾醇含量进行测定。该方法在2.0~20.0μg/m L线性范围内具有良好的线性关系,相关系数为0.999 6;以胆固醇作替代内标,平均加标回收率为95.7%~101.4%,相对标准偏差为1.0%~3.7%;方法的检出限为0.2 mg/kg。该方法操作简便,检测灵敏度高,干扰小,适用于食用植物油中β-谷甾醇含量的检测。A method for the determination of β- sitosterol in edible vegetable oil was established by gaschromatography- tandem mass spectrometry（ GC- MS / MS）. After the oil sample was saponified by 2mol / L potassium hydroxide- ethanol solution and the unsaponifiable fractions were extracted by petroleum ether,β- sitosterol could be analyzed by gas chromatography- tandem mass spectrometry in multiple reaction monitoring mode. There was a good linear relationship in the range of 2. 0- 20. 0 μg / m L. The correlation coefficient,the recovery of cholesterol,the relative standard deviation and the limit of detection of the method were 0. 999 6,95. 7%- 101. 4%,1. 0%- 3. 7% and 0. 2 mg / kg,respectively. The method had the items of simpleness and sensitiveness,which was applicable to determine the content ofβ- sitosterol in edible vegetable oil.

关 键 词：气相色谱-串联质谱法 Β-谷甾醇 胆固醇 食用植物油 

分 类 号：O657.63[理学—分析化学] TS225.1[理学—化学]