食管鳞癌中RhoE与EGFR表达及相关性的研究  

The Study of the Expression and Correlation of RhoE and EGFR in Esophageal Squamous Cell Carcinoma

作　　者：王豪杰[1] 施巩宁[1] 赵辉[1] 梁冰[1] 车建波[1] 王一[1] 

机构地区：[1]河南大学淮河医院,河南开封475000

出　　处：《中国医学创新》2015年第6期5-8,共4页Medical Innovation of China

基　　金：河南省教育厅科学技术研究重点项目(14A320081)

摘　　要：目的:探讨Rho E与EGFR在食管鳞状细胞癌中的表达及相关性,并分析其临床意义。方法:收集2011年1月-2012年1月本院的食管鳞状细胞癌癌组织及相应的癌旁组织59例,运用RT-PCR方法对进行检测,测定Rho E与EGFR的表达情况,结合患者其他指标运用统计学方法进行分析。结果:(1)在食管鳞癌和正常的食管组织中,EGFR相对的表达量分别是(0.581±0.174)、(0.347±0.136),差异有统计学意义(P<0.05)。(2)Rho E在食管鳞癌组织中的相对表达量是(0.346±0.241),在正常食管组织中相对的表达量为(0.673±0.142),差异有统计学意义(P<0.05)。(3)两者在食管鳞癌组织中的表达水平与肿瘤的病理分期、分化程度的高低、淋巴有否转移以及肿瘤浸润情况都有着密切关系(P<0.001),但是与其他因素诸如肿瘤分型、大小和部位无关(P>0.05)。(4)Rho E与EGFR在食管鳞癌组织中的表达呈负相关(P<0.01)。结论:EGFR与Rho E两者在食管鳞癌中的表达呈负相关,EGFR基因的表达水平是增高的,而Rho E的表达的水平是降低的;两者的表达水平与肿瘤的病理分期、分化程度的高低、淋巴有否转移以及肿瘤浸润情况等多种生物学行为密切相关,由此可以看出,两者的表达水平在食管鳞癌的发生、发展中起着至关重要的作用;检测食管鳞癌组织中两者的表达情况有助于评估其预后。Objective：To explore the expression and correlation of Rho E and EGFR in esophageal squamous cell carcinoma, and analyze its clinical significance.Method：59 patients with esophageal carcinoma were collect from the in our hospital from Jan 2011 to Jan 2012. The method of RT-PCR was used to examine the expressions of Rho E and EGFR in esophageal carcinoma and normal esophageal tissues. The results and other relevant clinical parameters were used for statistical analysis.Result：（1）The relative expression of EGFR in esophageal squamous cell carcinoma and normal esophageal tissues were（0.581±0.174） and（0.347±0.136）,the difference between them was significant（P〈0.05）.（2）The relative expression of Rho E in esophageal squamous cell carcinoma and normal esophageal tissues were（0.346±0.241） and（0.673±0.142）, the difference between them was significant（P〈0.05）.（3）The expressions of them were closely related to expression of tumor pathological staging, differentiation degree, lymph metastasis and high tumor infiltration（P〈0.001）,but had no relationship with other factors such as tumor type, size and location（P〈0.01）.（4）It had a negative correlation between the expression of Rho E and EGFR in esophageal squamous cell carcinoma（P〈0.01）.Conclusion：The expression level of EGFR gene in esophageal squamous cell carcinoma is higher, while the Rho E expression level is lower; it has a negative correlation between them. The expressions of them have a closely relationship with the expression of tumor pathological staging, differentiation degree, lymph metastasis and high tumor infiltration.Thus,it can be seen that,the expressions of Rho E and EGFR plays a vital role in the occurrence and development of esophageal squamous cell carcinoma. Testing the expressions of esophageal squamous cell carcinoma tissue will help us to evaluate the prognosis.

关 键 词：食管鳞癌 EGFR RHO E RT-PCR 

分 类 号：R735.1[医药卫生—肿瘤]