UPLC/Q-TOF鉴定染料木素在小鼠血浆中的代谢产物  

Determination of Genistein Metabolites in Plasma of Mice by UPLC/Q-TOF

作　　者：邢萌萌[1] 聂少平[1] 杨希[1] 杜国[1] 谢敏[1] 李文娟[1] 谢明勇[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047

出　　处：《南昌大学学报（理科版）》2014年第1期79-85,共7页Journal of Nanchang University(Natural Science)

基　　金：科技部国际合作项目(2010DFA31780)

摘　　要：建立一套稳定、灵敏度高、重现性好和耗时短的鉴定小鼠灌胃染料木素单体后体内代谢产物的分析方法,检测染料木素在小鼠血浆中代谢产物的形式与含量。12只健康雌性小鼠按200 mg·kg-1灌胃染料木素单体,血浆预处理处理后,使用超高效液相色谱(UPLC)/四极杆-飞行时间串联质谱仪(Q-TOF)检测,以体积分数2.5 mM乙酸铵缓冲液(A)-乙腈(B)为流动相梯度洗脱,电喷雾离子源负离子条件下进行MS1和MS2全扫描,鉴定小鼠给药染料木素单体后血浆中的代谢产物。染料木素在小鼠血液中的代谢产物的主要存在形式是染料木素-葡糖醛酸,约占54.82%;其次是染料木素-硫酸酯,约占26.67%;也有一小部分(16.61%)以染料木素苷元的形式存在。UPLC/Q-TOF可快速、有效地鉴定出小鼠血浆中染料木素的代谢产物,为今后进行染料木素与人类健康效应相关的研究提供参考。The purpose of this research was to develop a stable,sensitive and reproducible method to qualitatively and quantitatively analyze genistein in blood samples of mice. 12 healthy female mice were administered by gavage with genistein at the dose of 200 mg·kg^-1body weight. The blood samples were analyzed by tandem quadrupoletime of flight mass spectrometry in a negative ion mode based on gradient elution with a mobile phase composed of 100% aqueous buffer（ 2. 5 mM ammonium acetate,A） and 100% acetonitrile（ B） to identify the metabolites of genistein after treatment. The data suggested that the main form of genistein in the blood samples of the genistein group were genistein-glucuronides（ accounting for 54. 82%）,genistein-sulfates（ 26. 67%）,and there also existed a small portion（ 16. 61%） of genistein aglycone. Meanwhile,these metabolites were not detected in the blood samples of control group. Our results indicated that UPLC / Q-TOF could quickly and effectively identify genistein metabolites in mice plasma and thus provide a novel reference for the investigation the human health effects of dietary genistein.

关 键 词：染料木素 四极杆-飞行时间串联质谱仪 代谢物 染料木素-葡糖醛酸 染料木素-硫酸酯 

分 类 号：R151.2[医药卫生—营养与食品卫生学] R969.1[医药卫生—公共卫生与预防医学]