HPLC-UV-CL联用研究霍山石斛生物碱清除自由基活性  

Cleaving hydroxyl radicals activity of the alkaloids from Dendrobium huoshanense C.Z.Tang et S.J.Cheng detected by on-line HPLC-UV-CL system

作　　者：陈乃东[1,2,3] 李俊[3] 金晖[1] 陈琼[1] 朱庆杰[1] 

机构地区：[1]皖西学院生物与制药工程学院,安徽六安237012 [2]皖西中药与天然药物工程技术研究中心,安徽六安237012 [3]安徽医科大学药学院,安徽合肥230032

出　　处：《食品工业科技》2015年第7期276-280,共5页Science and Technology of Food Industry

基　　金：国家自然科学基金(81274021);中国博士后基金(2014M551791);安徽省人事厅博士后研究项目;安徽高校省级科学研究重点项目(KJ2012A277);六安市定向委托皖西学院市级研究重点项目(2011LWA001);皖西学院研究性学习项目(WXXYX2013063;WXXYX2013055;WXXYX2013080)

摘　　要：目的:霍山石斛生物碱抗氧化活性成分在线测定,为霍山石斛药效物质基础研究及抗氧化活性物质快速发现提供依据;方法:在预实验基础上建立霍山石斛生物碱部位HPLC-UV分析方法,采用鲁米诺-双氧水发光体系作为羟自由基供体、以校正清除率为抗氧化活性评价标准,HPLC-UV-CL联用在线测定霍山石斛生物碱部位各组分清除羟自由基活性。以磷钼酸、碘-碘化钾为沉淀试剂敲出总生物碱提取物中生物碱组份,对比敲出前后HPLC图谱变化,确定霍山石斛的生物碱组分。结果:在本实验条件下,霍山石斛中检出4种生物碱峰6、峰10、峰11、峰14,其中峰14、峰11具有一定的清除羟自由基活性,校正清除率达4.95%和10.94%。峰6、峰10对鲁米诺-双氧水系统发光值无明显影响。结论:HPLC-UV-CL可用于霍山石斛生物碱清除自由基活性快速测定。Objective：To explore the on-line detecting on the anti-oxidant activity of the alkaloids from the stems of Dendrobium huoshanense C. Z. Tang et S. J. Cheng by HPLC- UV- CL system, and provide references on researching the potency material base of D.huoshanense and on quick discovery of antioxidants from Traditional Chinese Medicine. Method. Applying the on- line HPLC - UV- CL analysis with Luminol- perhydrol chemiluminescence system as the donor of hydroxyl radicals to evaluate the anti- oxidant activity of the constituents of the total alkaloid extraction from D.huoshanense.The alkaloids were identified by knocking-out the alkaloid components using phosphomolybdic acid and iodine-potassium iodide as precipitation reagent.Result. Total four kinds of D.huoshanense alkaloids（the HPLC peak 6, peak 10, peak 11 and peak 14） were checked out and peak ]4 and peak 11 showed remarkable hydroxyl-radical-cleaning activity with the calibration clearance values 10.94% and 4.95 %, respectyively.No effect of the other two D.huoshanense alkaloids （ peak 6 and peak !0） on the chemiluminescence value of Luminol-perhydrol system were determined by the established on-line HPLC- UV-CL method. Conclusion ：The on-line HPLC-UV-CL system could be used to evaluate the cleaving hydroxyl radicals activity of D.huoshanense alkaloids.

关 键 词：霍山石斛 高效液相-化学发光联用 清除羟自由基活性 生物碱 

分 类 号：TS201.2[轻工技术与工程—食品科学]