红花油体提取条件优化及稳定性研究  

Optimization of Extract Condition of Safflower Oil Body and Analysis of Stability

作　　者：杨晶[1,2] 韩高强[2] 刘忠良[2] 卢振[2] 吴治邦[2] 王清曼 邹德毅 强卫东[2] 陈伟[2] 

机构地区：[1]吉林农业大学生物反应器与药物开发教育部工程研究中心,长春130118 [2]吉林农业大学生命科学学院,长春130118

出　　处：《生物技术通报》2015年第3期185-190,共6页Biotechnology Bulletin

基　　金：十二五国家高新技术研究与发展计划项目("863"计划项目);2012年国家大学生创新创业项目(2011AA100606);国家教育部博士点基金-青年教师基金项目(20122223120002);吉林省中医药管理局项目(2012162);吉林省教育厅项目(201458)

摘　　要：油体是储藏脂肪的亚细胞单位,其表面包裹一层磷脂和油体蛋白。这种稳定的结构可以保护油体面对环境的压力,使油体可以应用在食品、化妆品及制药工业中。研究红花油体的提取方法及红花油体乳液的基本性质,旨在为以油体为基质的载体体系研究奠定基础。以pBS为介质,采用梯度离心法,比较了不同提取条件对红花油体的提取效率的影响;对其在不同pH值、NaCl浓度条件下红花油体的平均粒径和稳定性进行测定。结果表明,红花油体在pH值≥6条件下,平均粒径为1.75-2.05μm和p H值≤6条件下,平均粒径1.50-1.75μm;NaCl浓度0.2和0.4 mg/m L时,红花油体分散较为均匀,NaCl浓度1.2和2.0 mg/m L时,红花油体出现聚集现象。蔗糖浓度0.1和0.2 mg/m L时,红花油体分散较为均匀,蔗糖浓度为0.4-1.0 mg/m L时,红花油体比较密集,随着蔗糖浓度的增加,红花油体的粒径逐渐开始不均一。红花油体的最佳提取条件是pH7,NaCl浓度0.2 mg/m L,蔗糖浓度0.1 mg/m L,稀释后的红花油体溶液在不加入保护剂或者不经过物理方法处理下,保存起来不稳定。In order to achieve a wide range of applications in many fields, especially on oil body as a substrate carrier system of the foundation, extraction method of safflower oil and characteristics of safflower oil body lotion was researched. Extraction efficiency of safflower oil body was compared, including its value at different pH, NaCl concentration conditions, an average particle size of safflower oil and stability, as well as its viscosity to conduct investigations. Results showed that the safflower oil body dispersed more evenly, at pH≥6, the average particle size of 1.75-2.05 μm and p H ≤ 6 conditions, the average particle size of 1.50-1.75 μm; while NaCl concentration of 0.2 and 0.4 mg/m L, NaCl concentration 1.2 and 2.0 mg/m L, the safflower oil bodies appeared aggregation. When sucrose concentration between 0.1 and 0.2 mg/m L, safflower oil dispersion is uniform; when the sugar concentration of 0.4-1.0 mg/m L, safflower body intensive, with increasing concentration of sucrose, safflower oil body size gradually began to heterogeneity. The optimum extraction condition for safflower oil body is pH7, NaCl 0.2 mg/m L, sucrose concentration of 0.1 mg/m L. The study proved that safflower oil body without adding detergent or without physical approach, the saved is unstable.

关 键 词：红花 油体 提取 粒径 稳定性 

分 类 号：TQ464[化学工程—制药化工]