谷氨酰胺转氨酶菌株的筛选及其产酶条件研究  

Screening of Microbial Transglutaminase Producing Strain and Optimazation of Fermentation Conditions

作　　者：宋小平[1] 王雅洁[1] 王迎新[1] 沈书文 李光伟[3] 

机构地区：[1]安徽医学高等专科学校药学系,安徽合肥230601 [2]合肥天麦生物科技发展公司,安徽合肥230031 [3]中国科技大学生物工程中试基地,安徽合肥230022

出　　处：《食品研究与开发》2015年第1期114-117,共4页Food Research and Development

基　　金：安徽省教育厅自然科学研究重点项目(KJ2010A201);安徽省专业带头人培养资助项目(皖教秘人[2013]189号)

摘　　要：利用蛋白质交联-絮凝沉淀性能测定方法,从土壤中分离得到1株高产谷氨酰胺转氨酶(microbial transglutaminase,MTG)的菌株,命名为HF-82,初步确定为链霉菌属。单因素试验确定最适产酶氮源和碳源分别是蛋白胨和葡萄糖,正交试验确定最适发酵培养基为(g/L):葡萄糖25.0,蛋白胨20.0,酵母提取物5.0,Mg SO4·7 H2O 2.0,K2HPO4·3 H2O 2.0,Na H2PO42.0,Ca CO33.0,p H7.0。此发酵工艺条件下,MTG的酶活可达到0.53 U/m L。A high transglutaminase-producing strain was isolated through a protein cross-linked-flocculation precipitate method, which was identified primarily to be a species of Streptomyces sp. The single factor experiments showed that the optimal carbon and nitrogen sources were glucose and peptone. The optimum medium composition was glucose25.0 g/L,Peptone 20.0 g/L,Yeast extract 5.0 g/L,MgSO4·7H2O 2.0g/L, K2HPO4·3H2O 2.0 g/L,NaH2PO4 2.0 g/L,CaCO3 3.0g/L received by Orthogonal Experimental Design. Under this fermentation process,the enzyme activity was promoted to 0.53 U/mL.

关 键 词：蛋白质交联-絮凝沉淀法 谷氨酰胺转氨酶 菌种选育 产酶条件 

分 类 号：TQ925[轻工技术与工程—发酵工程]