宁夏不同品种干红葡萄酒中辅色素的构成及定量分析  

Quantitative & Qualitative Analysis of Copigments in Dry Red Wine Made by Different Varieties of Grape from Ningxia

作　　者：王宏[1] 张军翔[1] 陈晓艺[1] 牛雪[1] 

机构地区：[1]宁夏大学农学院,宁夏银川750021

出　　处：《酿酒科技》2015年第3期115-119,共5页Liquor-Making Science & Technology

基　　金：2013年度国家自然科学地区科学基金项目(编号:31260392)

摘　　要：利用高效液相色谱法（HPLC）对宁夏贺兰山东麓赤霞珠、蛇龙珠、梅辘辄、西拉及黑比诺5个品种的干红葡萄酒进行辅色素的定性定量分析，研究宁夏不同单品种红葡萄酒中初始辅色素的构成及含量。结果表明，在色谱条件为：ZORBAXSB C18柱（250 mmx4.6 mm，5μm）；流动相A：水∶乙酸=98∶2（v/v），流动相B：乙腈；梯度洗脱程序：0 10 min，16%B；10 25 min，20% 40%B；25 30 min，40% 0%B；柱温箱温度为30℃；流速为1.0 mL/min，进样量为10μL，检测波长为280 nm条件下各个单体酚在30 min内得到了较好的分离。5种干红葡萄酒均被检测出没食子酸、安息香酸、儿茶素、香豆酸、丁香酸、水杨酸、阿魏酸、咖啡酸、芦丁和槲皮素10种重要辅色素，其中，赤霞珠和梅辘辄10种辅色素总含量很接近，都在1300 mg/L左右；其余3个品种的含量都在950 mg/L左右。对不同干红葡萄酒中的辅色素进行主成分分析，确定了葡萄酒中不同辅色素的贡献率，并得到3个主成分和10种辅色素的线性组合关系。In this study, qualitative＆amp;quantitative analysis of copigments in dry red wine made by five varieties of grape from Ningxia （Caber-net Sauvignon, Cabernet Gernischt, Merlot, Syrah and Pinot Noir from east foot of Helan Moutain） were done to know the initial composition and content of copigments in dry red wine. The experimental results showed that, the chromatographic conditions were ZorbaxSB-C18 column （250 mm x 4.6 mm, 5 μm）; mobile phase A： water∶acetic acid = 98∶2 （v/v）; mobile phase B： acetonitrile; gradient elution procedures： 0-10min, 16%B, 10-25 min, 20%-40%B, and 25-30 min, 40%-0%B;column temperature at 30℃;flow rate was 1.0 mL/min, and in-jection volume was 10μL and the detective wavelength was 280 nm. Under the above conditions, various phenolic compounds were separated successfully. 10 kinds of important copigments including gallic acid, benzoic acid, catechin, coumalic acid, syringic acid, salicy acid, ferulic ac-id, caffeic acid, rutin hydrate and quercetin were detected in all five wine samples. The total content of the 10 kinds of copigments was about 1300 mg/L in wine made by Cabernet Sauvignon/Merlot and about 950 mg/L in wine made by other three grape varieties. After principal com-ponent analysis of the copigments in different red wines, the contribution rate of different copigment in grape wine was determined and the lin-ear combination relationship between three principal components and the 10 kinds of copigments were obtained.

关 键 词：辅色素 高效液相色谱 干红葡萄酒 主成分分析 

分 类 号：TS262.6[轻工技术与工程—发酵工程]