外源中性蛋白酶对猪粪液臭味物质的影响  

Effect of Exogenous Neutral Protease on Odor Substances in Swine Liquid Manure

作　　者：杨林青 吴月华 盛清凯 王天惠 李彦英 韩云海 

机构地区：[1]山东省思威化学品安全评价中心,山东济南250014 [2]山东省农科院畜牧兽医研究所/山东省畜禽疫病防治与繁育重点试验室,山东济南250100 [3]山东奥博生物科技有限公司,山东高唐252800

出　　处：《山东化工》2015年第6期171-173,共3页Shandong Chemical Industry

基　　金：国家自然科学基金项目(31172245)

摘　　要：为了减少臭味污染,研究外源中性蛋白酶对猪粪液中氨、硫化氢、粪臭素及相关酶的影响。猪粪液中添加枯草芽孢杆菌发酵。发酵24h。结果为中性蛋白酶降低了猪粪发酵液OD值(p<0.01),以及p H值(p<0.05),提高了色氨酸浓度和蛋白酶活性(p<0.05)。硫化氢、粪臭素浓度及纤维素酶活性降低,但差异不显著。结果显示枯草芽孢杆菌发酵猪粪液中添加外源中性蛋白酶有助于臭气减排。In order to reduce odor pollution, This study investigated the effect of exogenous neutral protease on ammonia, hydrogen sulfide, skatole and related enzymes in Swine Liquid Manure. Bacillus subtilis was added in liquid manure and the fermentation period lasted 24h. The results were that neutral protease reduced the liquid manure OD value （P 〈0.01 ）, and pH （P 〈 0.05 ）, improved the tryptophan concentration and protease activity （ P 〈 0. 05 ）. The concentrations of hydrogen sulfide, skatole and cellulase activity descended （ P 〉 0.05 ）. The results showed Adding exogenous neutral proteasc in pig liquid manure fermented by Bacillus subtilis contributes to the reduction of odor emission.

关 键 词：枯草芽孢杆菌 粪液 中性蛋白酶 臭气 

分 类 号：TQ925.2[轻工技术与工程—发酵工程]