hTERT与SV40 LT协同表达对牛乳腺上皮细胞体外增殖的影响  

Cell Proliferation Effect Co-expression of hTERT and SV40 LT Gene on Bovine Mammary Gland Epithelial Cells In Vitro

作　　者：贺小英[1] 马利兵[1] 程腾[1] 

机构地区：[1]内蒙古科技大学数理与生物工程学院,包头014010

出　　处：《中国细胞生物学学报》2015年第2期197-203,共7页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:31160245);内蒙古自然科学基金(批准号:2012MS0503);内蒙古自治区高等学校青年科技英才支持计划资助的课题~~

摘　　要：人端粒酶催化亚基(human telomerase reverse transcriptase,h TERT)和SV40大T抗原(simian virus 40 large antigen,SV40 LT)均能刺激细胞增殖并延长细胞体外寿命,但相对效率较低,二者协同能否增强功效有待于进一步研究。该文首先构建了h TERT和SV40 LT双基因共表达载体,转染牛乳腺上皮细胞(bovine mammary gland epithelial cells,b MGEs),获得阳性克隆。持续扩大培养时,细胞活力旺盛,且细胞能体外维持寿命超过30代。RT-PCR和Western blot检测结果表明,h TERT和SV40 LT能同时表达。流式细胞仪检测发现,阳性细胞具有较强的增殖能力。研究结果证实,h TERT和SV40 LT协同表达能刺激b MGEs体外增殖及延长细胞寿命。Human telomerase reverse transcriptase（h TERT） and simian virus 40 large antigen（SV40 LT） can stimulate cell proliferation and extend cell life-span, however, they are low efficient. Therefore, it remains for further research to verify whether the two genes have co-expression patterns. In this study, the co-expression plasmid carrying h TERT and SV40 LT gene was constructed and transfected bovine mammary epithelial cells（b MGEs）. The obtained positive cells could maintain growth through passage 30. Using RT-PCR and Western blot, the expressions of h TERT and SV40 LT were detected in positive cells. Furthermore, there were higher proliferation rates in the positive cells than those of control group by fl ow cytometer. The results showed that the co-expression of h TERT and SV40 LT might stimulate cell proliferation and extend cell life-span.

关 键 词：端粒酶 SV40 乳腺细胞 增殖 协同表达 

分 类 号：Q813.11[生物学—生物工程]