肾透明细胞癌N-WASP蛋白表达特点及与临床病理相关性  

Protein expression pattern and correlation with clinicopathological features in clear cell renal cell carcinoma neural Wiskott-Aldrich syndrome

作　　者：陈健[1] 纪志刚[1] 刘广华[1] 周立[2] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院泌尿外科,北京100730 [2]中国医学科学院北京协和医学院北京协和医院基本外科,北京100730

出　　处：《基础医学与临床》2015年第3期366-370,共5页Basic and Clinical Medicine

摘　　要：目的研究肾透明细胞癌组织及癌旁正常肾组织中N-WASP表达特点及与临床病理的相关性。方法对73例肾透明细胞癌组织及癌旁正常肾组织行N-WASP免疫组织化学染色,比较两组表达差异。对癌组织N-WASP表达水平与肿瘤病理分级,临床分期进行相关性分析。对总体生存率行单因素及多因素预后分析。结果癌组织N-WASP表达低于癌旁组织表达(P<0.001)。癌组织中N-WASP低、中表达的患者生存率高于高表达患者(P<0.01)。癌组织N-WASP表达水平(P<0.01)与患者总体生存率相关。癌组织N-WASP表达水平可以作为判断肾透明细胞癌预后的独立预测因素(P<0.05)。结论 N-WASP在肾透明细胞癌中表达明显下降,并可以作为独立预测因素提示患者预后。Objective To examine the level of N-WASP expression in clear cell renal cell carcinoma（ CCRCC）and its association with clinicopathological features. Methods Immunohistochemical staining for N-WASP was performed on tissue microarrays constructed from tumor and para-tumor tissue obtained from 73 CCRCC patients. The difference in N-WASP expression between tumor tissue and adjacent normal renal tissue was examined. Correlations between N-WASP expression in the tumor and clinicopathological parameters were analyzed and the relationship between N-WASP expression and overall survival also assessed. Univariate and multivariate survival analyses were performed using the Cox proportional hazards regression model. Results N-WASP expression was reduced in tumor tissues（ P 0. 001）. Patients with weak-to-moderate N-WASP expression tended to have a longer over-all survival than those with a high tumor N-WASP level（ P 0. 01）. A higher level of N-WASP expression in the tumor was an indicator of poor survival in CCRCC patients（ P 0. 01）. N-WASP was an independent predictor for overall survival（ P 0. 05）. Conclusions N-WASP was significantly downregulated in CCRCC and could serve as a promising prognostic biomarker for predicting clinical outcome.

关 键 词：肿瘤指标 肾透明细胞癌 N-WASP 组织芯片 

分 类 号：R737.11[医药卫生—肿瘤]