大蒜素对脑胶质瘤细胞U87细胞侵袭能力的影响及其机制  

The effect and mechanism of allicin on the migration and invasion properties of human osteosarcoma U87 cells

作　　者：蔡青[1] 秦怀洲[1] 陈昆仑[2] 

机构地区：[1]第四军医大学唐都医院神经外科,陕西西安710038 [2]西安交通大学医学部第二附属医院普外科,陕西西安710004

出　　处：《西安交通大学学报（医学版）》2015年第2期271-274,284,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的检测大蒜素对脑胶质瘤细胞U87侵袭能力的影响及其可能机制。方法利用MTT法检测大蒜素对U87细胞增殖能力的抑制作用。利用Transwell小室检测大蒜素对U87细胞侵袭能力的抑制作用。采用Real-time PCR和Western blotting方法检测大蒜素对U87细胞中MMP-2和MMP-9表达水平的影响。采用Western blotting的方法检测大蒜素对p38磷酸化水平的影响。结果大蒜素能够明显抑制U87细胞的增殖(浓度>8μg/mL,P<0.05)。且大蒜素(浓度<8μg/mL)能够明显抑制U87细胞的侵袭能力(P<0.05)。在大蒜素作用24h后,U87细胞中MMP-2和MMP-9的表达水平明显降低(P<0.05)。p38的磷酸化水平在大蒜素作用后明显降低(P<0.05)。结论大蒜素能够抑制脑胶质瘤细胞U87的侵袭能力,其机制可能是通过对p38信号的通路活性的调节进而降低MMP-2和MMP-9的表达来实现的,提示大蒜素在脑胶质瘤的治疗中具有潜在的应用价值。Objective To investigate the anti-metastatic effect of allicin on glioma cell line U87 and related mechanisms.Methods In this study,we employed MTT assay to test the anti-proliferative effect of allicin. Transwell assay was used to test the anti-metastatic ability of allicin.Real-time PCR and Western blotting were employed to test the effect of allicin on the expressions of matrix metalloproteinase-2 （MMP-2 ） and matrix metalloproteinase-9 （MMP-9）.Western blotting was employed to test the phosphorylated level of p38.Results Allicin could significantly inhibit the proliferation and invasion of U87 cells （concentration＆gt;8 μg/mL,P 〈0.05）. Meanwhile allicin （concentration〉8μg/mL）could inhibit the invasion of U87 cells.After treatment with allicin for 24 hours,the expressions of MMP-2 and MMP-9 were decreased significantly （P 〈 0.05 ）.Moreover,allicin treatment decreased the phosphorylated level of p38 obviously （P 〈 0.05 ）.Conclusion Allicin inhibits the invasion and migration of glioma cell line U87 by reducing the expressions of MMP-2 and MMP-9 via suppressing the activity of p38 signal pathway,suggesting that allicin is a potential therapeutic agent for glioma.

关 键 词：脑胶质瘤 大蒜素 侵袭 MMPS P38 U87细胞 

分 类 号：R739.41[医药卫生—肿瘤]