珊瑚状猴头菌多糖抗氧化作用研究  

Studies on Polysaccharides from Hericium coralloids on the Antioxidant Activity in Mice

作　　者：田敏[1] 王荣荣[2] 冯翠萍[1] 常明昌 程红艳[4] 孟俊龙[1] 

机构地区：[1]山西农业大学食品科学与工程学院,山西太谷030801 [2]信阳农林学院生物技术系,河南信阳464000 [3]山西省食用菌工程技术研究中心,山西太谷030801 [4]山西农业大学资源与环境学院,山西太谷030801

出　　处：《中国食品学报》2015年第1期25-33,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：山西省自然科学基金项目(2010011043-1);山西省科技基础条件平台建设项目(2010091017);山西省科技攻关项目(20130311017-3)

摘　　要：目的:探讨珊瑚状猴头菌多糖的抗氧化作用。方法:体外抗氧化活性以测定其总还原力,对DPPH自由基、O2-·、·OH的清除率,抑制体外脂质过氧化能力为指标;体内抗氧化试验:将48只昆明小鼠,雌、雄各半,随机分为对照组、低剂量组【100 mg/(kg·d)】、中剂量组【200 mg/(kg·d)】、高剂量组【400 mg/(kg·d)】4个组。灌胃30d后,采样,测定小鼠血清、心脏、肝脏、肾脏组织中总抗氧化能力、超氧化物歧化酶、谷胱甘肽过氧化物酶、过氧化氢酶活力及丙二醛含量。结果:随着珊瑚状猴头菌多糖质量浓度的增加,其抗氧化能力增强;当其质量浓度达到2 mg/m L时,还原力吸光度为0.482,对DPPH自由基、O2-·和·OH的清除率分别为80.10%,91.72%和94.33%,脂质过氧化物抑制率为68.71%;可提高心脏、肝脏、肾脏等组织及血清中T-AOC、SOD、GSH-Px和过氧化氢酶活力,降低MDA含量,在高剂量时差异达极显著水平(P<0.01)。结论:珊瑚状猴头菌多糖具有抗氧化作用。Object： To investigate the effects of polysaccharides from Hericium coralloids. Methods： Antioxidant activity of Hericium coralloids polysaccharide in vitro is determined by the total reducing power, the scavenging capacity of DPPH·, superoxide anion radical（O2^-·）, hydroxyl radical（·OH） and lipid oxidation inhibiting activity; Experiment in vivo, 48 healthy mice were randomly divided into control group, low dose group（100 mg/kg）, middle dose group（200mg/kg） and high dose（400 mg/kg）（female∶male=1∶1）. After 30 d, the levels of MDA and the activity of T-AOC, SOD,CAT, GSH-PX in serum, heart, liver, kidney were examined. Results： Hericium coralloids polysaccharide has certain antioxidant activity. Values of the ability of antioxidant increased with increasing the concentrations of polysaccharide. At2 mg/ml, the absorbance of reducing powers was 0.482, DPPH·、 O2^-·and ·OH scavenging rate, and lipid oxidation inhibiting activity were 80.1%, 91.72%, 94.33% and 68.7% respectively. Hericium coralloids polysaccharide can increase the contents of T-AOC, SOD, GSH-Px, CAT and decrease the levels of MDA in organ and serum, and the difference were significant in high dose（P〈0.01）. Conclusion： It was indicated Hericium coralloids polysaccharides had antioxidant activity.

关 键 词：珊瑚状猴头菌 自由基 抗氧化 

分 类 号：R151[医药卫生—营养与食品卫生学]