Simultaneous Determination of Five Hydrophilic and Lipophilic Components from roots of Salvia miltiorrhiza by HPLC  

作　　者：Li-lan Lu Shuang Hou Ting-ting Zheng Xing-li Zhang Jian-he Wei 

机构地区：[1]Hainan Branch of Institute of Medicinal Plant Development(Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine),Chinese Academy of Medical Sciences [2]Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences

出　　处：《Chinese Herbal Medicines》2015年第1期75-79,共5页中草药（英文版）

基　　金：National Department Public Benefit Research Foundation(201107011)

摘　　要：Objective A reversed-phase HPLC method was established for the simultaneous determination of five hydrophilic and lipophilic components in the roots of Salvia miltiorrhiza. Methods Hydrophilic components including danshensu, protocatechuic aldehyde, and salvianolic acid B, and lipophilic components such as cryptotanshinone and tanshinone liA, were successfully separated on a Waters Symmetry C18 reverse- phase column （250 mm×4.6 mm, 5 μm）, with acetonitrile-0.5% phosphoric acid （gradient elution） as mobile phase, the detection wavelength was set at 281 nm with flow rate of 1.0 mL/min, and the column temperature was maintained at 30 ~C. Results The recovery of the method was in the range of 95.1%-102.5% and the precision was less than 3% for all five analytes. All the compounds showed good linearity （R2 〉 0.9990） in a relatively wide concentration range. Therefore, this HPLC method demonstrated good reproducibility, stability, and accuracy in validation studies. Conclusion Simultaneous quantification of the multiple components by HPLC would be a better strategy for the quality evaluation on the roots of S. miltiorrhiza.Objective A reversed-phase HPLC method was established for the simultaneous determination of five hydrophilic and lipophilic components in the roots of Salvia miltiorrhiza. Methods Hydrophilic components including danshensu, protocatechuic aldehyde, and salvianolic acid B, and lipophilic components such as cryptotanshinone and tanshinone liA, were successfully separated on a Waters Symmetry C18 reverse- phase column （250 mm×4.6 mm, 5 μm）, with acetonitrile-0.5% phosphoric acid （gradient elution） as mobile phase, the detection wavelength was set at 281 nm with flow rate of 1.0 mL/min, and the column temperature was maintained at 30 ~C. Results The recovery of the method was in the range of 95.1%-102.5% and the precision was less than 3% for all five analytes. All the compounds showed good linearity （R2 〉 0.9990） in a relatively wide concentration range. Therefore, this HPLC method demonstrated good reproducibility, stability, and accuracy in validation studies. Conclusion Simultaneous quantification of the multiple components by HPLC would be a better strategy for the quality evaluation on the roots of S. miltiorrhiza.

关 键 词：CRYPTOTANSHINONE DANSHENSU HPLC determination protocatechuic aldehyde Salviamiltiorrhiza salvianolic acid B tanshinone IIA 

分 类 号：R284[医药卫生—中药学] O657.72[医药卫生—中医学]