新疆双峰驼乳清蛋白组分对人宫颈癌HeLa细胞增殖的抑制作用  

Inhibition of Cell Proliferation of Active-protein Fraction from Bactrian Camel Whey on Cervical Cancer He La Cells in vitro

作　　者：杨洁[1] 王红娟[1] 豆智华[1] 李冠[1] 

机构地区：[1]新疆大学生命科学与技术学院,新疆乌鲁木齐830046

出　　处：《新疆大学学报（自然科学版）》2015年第1期11-16,共6页Journal of Xinjiang University(Natural Science Edition)

基　　金：国家自然科学基金(31260369);新疆维吾尔自治区科技援疆项目(201191255)

摘　　要：目的:探讨新疆双峰驼乳清蛋白组分对人宫颈癌HeLa细胞增殖的抑制作用.方法:采用四甲基偶氮唑盐(MTT)法测定新疆双峰驼乳蛋白组分对HeLa细胞增殖的抑制作用;荧光染色检测HeLa细胞凋亡的形态变化;倒置显微镜、扫描电镜和透射电镜观察HeLa细胞的形态变化;用流式细胞术检测HeLa细胞凋亡率、线粒体膜电位的变化.结果表明:新疆双峰驼乳清蛋白组分TR35可抑制人宫颈癌HeLa细胞的增殖并诱导其凋亡,并呈时间和剂量依赖性(P<0.01);倒置显微镜、扫描电镜和透射电镜观察到典型的凋亡特征,荧光显微观察到凋亡小体;Annexin-Ⅴ/PI双染检测出现早期凋亡细胞;线粒体膜电位去极化,并将细胞周期阻滞在S期.结论:新疆双峰驼乳清蛋白组分TR35可抑制HeLa细胞的增殖和诱导细胞凋亡,TR35组分诱导细胞凋亡的途径可能与线粒体通路有关.To explore the Xinjiang bactrian camel milk suppressor component that inhibit tumor cell proliferation in vitro and its mechanism, we adopt MTT to screen the whey protein components with anticancer activity. Hela cell morphological changes were studied by inverted microscope, scanning electron microscopy（sem） and transmission electron microscopy and fluorescence microscopy. Flow cytometry was used to detect apoptosis rate, the change of mitochondrial membrane potential in the Hela cells after 24 h treatment with camel whey tumor-suppressor component. Results show that the TR35 fractionation from camel whey have a certain inhibitory effect on cancer cell, and had a certain amount of time-dose dependent manner. TR35 components can induce the apoptosis of Hela cells, the apoptotic body, mitochondrial membrane potential depolarization, and cell cycle arrest in S phase. Under the light microscope and electron microscope showed typical apoptosis characteristics. Annexin-Ⅴ/PI double dye detection appeared early apoptosis cells. Conclusion： Xinjiang bactrian camel whey protein components TR35 inhibits proliferation of esophageal Hela cell, and the induced way may be associated with mitochondrial pathway.

关 键 词：双峰驼 乳清蛋白 HELA 抗癌 细胞凋亡 

分 类 号：TS201.2[轻工技术与工程—食品科学]