广州市香港海鸥菌检测及耐药性分析  

Detection and drug resistance of Laribacter hongkongensis isolated from freshwater products and reservoir water in Guangzhou city

作　　者：王祉蕴 冯梅[1] 刘志华[1] 陈清[1] 黄建华[1] 陈少威[1] 钟雪珊[1] 胡静[1] 

机构地区：[1]南方医科大学流行病学系,广东广州510515

出　　处：《中国公共卫生》2015年第4期473-475,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(81373052)

摘　　要：目的了解广东省广州市淡水产品及水库香港海鸥菌污染的状况,并分析其耐药性。方法于2013年12月—2014年5月共采集广州市4家农贸市场3个不同品种淡水产品324份及6个水库水样6份,共计330份样本进行检测,检样接种改良头孢哌酮Mac Conkey琼脂（CMA）平板培养分离可疑菌株,经革兰染色、生化实验、特异性片段PCR扩增进行香港海鸥菌的鉴定,并选取11株菌株进行测序。采用K-B法进行药敏实验。结果 324份淡水产品经细菌培养共检出香港海鸥菌53株,总阳性率为16.36%（53/324）,其中香港海鸥菌在草鱼中阳性率为17.97%（46/256）,虎纹蛙中阳性率为46.67%（7/15）。采样6个水库中,仅于和龙水库检出1株香港海鸥菌。菌株测序结果与海鸥菌标准株HKU1相应序列的一致性达98%-99%。分离株对一、三代头孢菌素类抗生素耐药率为94.44%-98.15%,对利福平耐药率为70.37%。结论广州市淡水产品及水库水源中可能存在香港海鸥菌污染且多数分离株对头孢菌素类抗生素及利福平耐药。Objective To examine Laribacter hongkongensis（LH） contamination of freshw ater products and reservoir water in Guangzhou city,and to assess drug resistance of LH isolated. Methods A total of 324 freshw ater product samples from 4 farmers＇ markets and 6 surface water samples from 6 reservoirs were collected from December 2013 to May 2014. All the samples were plated onto cefoperazone Mac Conkey agar and incubated under aerobic condition at37 ℃ for 48 hours. All suspected isolates were identied phenotypically with standard conventional biochemical methods.Isolates suspected to be LH were subjected to 16 S rRNA gene sequencing. Results The overall detection rate of LH was16. 36%（53 /324） for freshw ater products,with the rates of 17. 97%（46 /256） for grass carps and 46. 67%（7 /15） for tiger frogs; but for 53 samples of Cipangopaludina cahayensis no LH strain was isolated. LH was isolated from Helong reservoir among six detected reservoirs. The genetic homology of the isolated LH strains with the standard strain（HKU1）w as 98%-99%. The rate of drug resistance of the isolated LH strains to first-generation cephalosporins and third-generation cephalosporins were 94. 44%-98. 15%,and 70. 37% to rifampicin. Conclusion There are contaminations of LH in freshw ater products and reservoir water in Guangzhou city and the LH strains are highly resistant to cephalosporin and rifampicin.

关 键 词：香港海鸥菌 淡水产品 水库 耐药性 

分 类 号：R155.5[医药卫生—营养与食品卫生学]