罂粟壳成分快速检测方法在食品监督中的应用  

Application of Fast Detection Metheds of Component of Poppy Shell in Food Supervision

作　　者：朱振华[1] 朱士新[1] 陈璐[1] 朱佳娴[1] 

机构地区：[1]昆山市卫生监督所,江苏昆山215300

出　　处：《中国食物与营养》2015年第3期8-10,共3页Food and Nutrition in China

基　　金：昆山市社会发展科技计划项目(项目编号:KS1355)

摘　　要：目的:建立食品中罂粟壳成分的快速检测方法,为罂粟壳成分的检测提供依据。方法:对监督执法中抽检的20份样品中的5种罂粟壳成分应用竞争抑制免疫层析快速检测法进行检测,然后利用液相色谱—质谱法对阳性样品进行检测验证。结果:经液相色谱—质谱法和竞争抑制免疫层析法检测,20份样品中有2份检测阳性、18份阴性,合格率90%,竞争抑制免疫层析法阳性检出率100%。结论:竞争抑制免疫层析法快速、便捷,干扰因素少,阳性检出率高,值得应用推广。【Objective】To establish a rapid method for compenents of poppy shell to provide reliable basis for the detection. 【Method】Totally 20 samples were detected by Competitive Inhibition Assay,and 2 positive samples were validated by Liquid Chromatography-Mass Spectrometry. 【Result】After Liquid Chromatography-Mass Spectrometry and Competitive Inhibition Assay,2 samples were in 20 samples,and the qualified rate was 90%,positive detection rate by the Competitive Inhibition Assay was 100%. 【Conclusion】The Competitive Inhibition Assay was rapid,convenient and less interference,positive detection rate was high,which was worth to be popularized in food supervision.

关 键 词：罂粟壳 快速检测方法 食品安全 液相色谱—质谱法 竞争抑制免疫层析法 

分 类 号：O657[理学—分析化学] TS207.3[理学—化学]