一株苯并芘高效降解菌的筛选鉴定  

Isolation and Identification of a Benzo Pyrence-Degrading Strain

作　　者：弓玉红[1] 李军[1] 高平[1] 王莉[1] 赵君[1] 

机构地区：[1]吕梁学院,山西离石033000

出　　处：《安徽农业科学》2015年第11期3-4,7,共3页Journal of Anhui Agricultural Sciences

基　　金：吕梁学院校级科技创新项目(20133061)

摘　　要：为了寻找可降解苯并芘的高效降解菌,从长期受苯并芘污染的焦化厂周边土壤和废水中取样,以苯并芘为唯一碳源反复驯化,分离、筛选出1株高效降解苯并芘的菌株A18。经形态特征和分子生物学分析,初步鉴定为木贼镰刀菌(Fusarium equiseti)。菌株A18在苯并芘溶液浓度为5 mg/L下,28℃振荡培养12 d,苯并芘的降解率达到44.8%。该菌株首次被证实具有降解苯并芘的能力。To find degrading strain of benzopyrene, sampled from the soil and waste water surrounding the coking plant which was polluted by bebzopyrene in a long time. An efficient degradation bacterium strain A18 was isolated from soil by repeated subculture in liquid medium with benzopyrence as sole carbon source. To confirm the classification of A18, morphological characteristics and molecular biology analysis were applied, the strain A18 was identified as Fusarium equiseti. In a mineral salts medium with 5 mg/L benzopyrence, the highest degradation rate of 44. 8% was obtained after 12 days at 28 ℃. The strain is for the first time confirmed has the ability to degrade the benzopyrene.

关 键 词：苯并芘 高效降解菌 筛选 鉴定 

分 类 号：S182[农业科学—农业基础科学] X172[环境科学与工程—环境科学]