茶多酚对鼻咽癌细胞增殖、凋亡、迁移和侵袭的影响及机制的研究  

Tumor suppressive effect and relative mechanisms of tea polyphenol on nasopharyngeal carcinoma cells

作　　者：田梦秋[1] 袁东杰[1] 郑实兴[1] 李庆玉[1] 石书婧 徐志文[1] 

机构地区：[1]广西医科大学第一附属医院耳鼻咽喉一头颈外科,南宁530021

出　　处：《临床耳鼻咽喉头颈外科杂志》2015年第6期552-556,共5页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：广西医疗卫生重点科研课题(No:重2012060)

摘　　要：目的:探索茶多酚对鼻咽癌细胞株HONE1增殖、凋亡、迁移和侵袭能力的影响,并研究相关的分子作用机制。方法:用不同浓度的茶多酚处理鼻咽癌HONE1细胞,分别用细胞计数法-8(CCK-8)法、荧光染色法、细胞划痕、Transwell侵袭实验、流式细胞术等方法,观察细胞生物学行为的变化;同时,检测茶多酚对鼻咽癌细胞中血管内皮生长因子(VEGF)表达的影响。结果:茶多酚能显著抑制鼻咽癌细胞HONE1的增殖,并呈浓度依赖性,而对正常鼻咽上皮细胞则无明显抑制作用;诱导HONE1细胞凋亡;茶多酚还有降低鼻咽癌细胞HONE1迁移和侵袭的能力,并明显减少鼻咽癌细胞内VEGF蛋白的表达。结论:茶多酚在体外能够抑制鼻咽癌细胞增殖,诱导细胞凋亡,并降低鼻咽癌细胞体外迁移和侵袭能力。Objective：To investigate the effect and mechanism of tea polyphenol（TP）on the proliferation,apoptosis,migration and invasion of nasopharyngeal carcinoma（NPC）cell line HONE1.Method：After treated with different concentration of tea polyphenol,CCK-8assay,fluorescent staining,cell scratching assay and transwell assay were applied to detect the effect of tea polyphenol on the HONE1 cells.Furthermore,the expression of protein VEGF was investigated by flow cytometry assay.Result：It was found that tea polyphenol could inhibit NPC cell proliferation significantly in a dose-dependent manner,however,little impact was observed in normal nasopharyngeal epithelial cell line NP69.Furthermore,it was demonstrated by fluorescent staining assay that tea polyphenol could induce NPC cell apoptosis,and cell scratching assay and transwell assay showed that tea polyphenol could inhibit cell migration and invasion.Conclusion：Tea polyphenol can significantly inhibit cell proliferation,induce cell apoptosis and decreased the migration and invasion ability of NPC cells in vitro.Tea polyphenol might be a tumor suppressor of NPC cells.

关 键 词：茶多酚 鼻咽癌 增殖抑制 转移 

分 类 号：R739.63[医药卫生—肿瘤]