红曲霉与何首乌双向发酵体系的探索  

Exploration of Co-fermentation System of Polygoni Multiflori Radix and Monascus

作　　者：谢炎福[1] 韩建明[1] 杜苗苗[1] 张优仪[1] 

机构地区：[1]洛阳师范学院,河南洛阳471022

出　　处：《中国实验方剂学杂志》2015年第8期17-20,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省自然科学基金项目(142102110173);洛阳师范学院应用基金项目(2012-10001410)

摘　　要：目的:分析红曲霉与何首乌双向液体发酵形成的药性基质主要活性成分,优选双向发酵工艺条件。方法:采用紫外-可见全波长扫描法和紫外分光光度法进行定性和定量分析,以二苯乙烯苷、结合蒽醌、游离蒽醌和莫纳可林K含量为评价指标,在单因素试验基础上,通过正交试验考察红曲霉接种量、何首乌添加量和发酵时间对红曲霉与何首乌双向液体发酵工艺的影响。结果:基质中二苯乙烯苷、结合蒽醌和总蒽醌含量呈下降趋势,而游离蒽醌含量上升,双向发酵最佳工艺条件为何首乌用量5%,发酵温度28℃,接种量6%,发酵时间7 d。二苯乙烯苷、结合蒽醌、游离蒽醌、莫纳科林K质量分数分别为37.84,0.89,1.54,0.41 mg·g-1。结论:何首乌对红曲霉的生长及其次级代谢产物莫纳科林K的积累具有促进作用,红曲霉降低了何首乌中结合蒽醌的含量,提高了何首乌的药用价值。Objective： To analyze main active compositions of drug-containing medium composed by monascus and Polygoni Muhiflori Radix after fermentation and optimize its fermentation technology. Method： Qualitative and quantitative analysis were established by UV-visible full wavelength scanning method and ultraviolet spectrophotometry, based on single factor tests, with yields of monacolin K, free-anthraquinone, stilbene glucoside and combined-anthraquinone as indexes, orthogonal test was adopted to investigate effects of fermentation time, amounts of monascus and Polygoni Multiflori Radix on fermentation technology. Result： Contents of stilbene glucoside, combined-anthraquinone and total anthraquinone showed a downward trend, but free-anthraquinone showed a upward trend. Optimum fermentation conditions were as follows： Polygoni Multiflori Radix amount of 5% , fermentation temperature at 28 ℃ , monascus amount of 6% , fermentation time of 7 d. Yields of stilbene glucoside, combined-anthraquinone, free-anthraquinone and monacolin K were 37.84, 0.89, 1.54, 0.41 mg ·g^-1, respectively. Conclusion： Polygoni Multiflori Radix has a promoting process on growth of monascus and its secondary metabolites of monacolin K, but monascus reduces the content of combined-anthraquinone in Polygoni Multiflori Radix, which improves medicinal value of Polygoni Multiflori Radix.

关 键 词：红曲霉 何首乌 双向发酵 二苯乙烯苷 莫纳科林K 

分 类 号：R283.6[医药卫生—中药学] R284.1[医药卫生—中医学]