Scutellarin attenuates endothelium-dependent aasodilation impairment induced by hypoxia reoxygenation, through regulating the PKG signaling pathway in rat coronary artery  

作　　者：CHEN Ya-Juan WANG Lei ZHOU Guang-Yu YU Xian-Lun ZHANG Yong-Hui HU Na LI Qing-Qing CHEN Chen QING Chen LIU Ying-Ting YANG Wei-Min 

机构地区：[1]School of Pharmaceutical Science & Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University [2]Zhaotong Institute of Tianma [3]Department of Periodontology and Implant Dentistry, The First People's Hospital of Yun-Nan Province [4]Kunhua Hospital Affiliated to Kunming University of Science and Technology

出　　处：《Chinese Journal of Natural Medicines》2015年第4期264-273,共10页中国天然药物（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.30960450,81173110);Yunnan Provincial Science and Technology Department(Nos.2011FA 022,2012BC012,2014FA010,2014FB037,2014BC012)

摘　　要：Scutellarin(SCU), a flavonoid from a traditional Chinese medicinal plant. Our previous study has demonstrated that SCU relaxes mouse aortic arteries mainly in an endothelium-depend-ent manner. In the present study, we investigated the vasoprotective effects of SCU against HR-induced endothelial dysfunction(ED) in isolated rat CA and the possible mechanisms involving cyclic guanosine monophosphate(c GMP) dependent protein kinase(PKG). The isolated endothelium-intact and endothelium-denuded rat CA rings were treated with HR injury. Evaluation of endothelium-dependent and-independent vasodilation relaxation of the CA rings were performed using wire myography and the protein expressions were assayed by Western blotting. SCU(10–1 000 μmol·L-1) could relax the endothelium-intact CA rings but not endothelium-denuded ones. In the intact CA rings, the PKG inhibitor, Rp-8-Br-c GMPS(PKGI-rp, 4 μmol·L-1), significantly blocked SCU(10–1 000 μmol·L-1)-induced relaxation. The NO synthase(NOS) inhibitor, NO-nitro-L-arginine methylester(L-NAME, 100 μmol·L-1), did not significantly change the effects of SCU(10–1 000 μmol·L-1). HR treatment significantly impaired ACh-induced relaxation, which was reversed by pre-incubation with SCU(500 μmol·L-1), while HR treatment did not altered NTG-induced vasodilation. PKGI-rp(4 μmol·L-1) blocked the protective effects of SCU in HR-treated CA rings. Additionally, HR treatment reduced phosphorylated vasodilator-stimulated phosphoprotein(p-VASP,phosphorylated product of PKG), which was reversed by SCU pre-incubation, suggesting that SCU activated PKG phosphorylation against HR injury. SCU induces CA vasodilation in an endothelium-dependent manner to and repairs HR-induced impairment via activation of PKG signaling pathway.Scutellarin （SCU）, a flavonoid from a traditional Chinese medicinal plant. Our previous study has demonstrated that SCU relaxes mouse aortic arteries mainly in an endothelium-depend-ent manner. In the present study, we investigated the vasopro- tective effects of SCU against HR-induced endothelial dysfunction （ED） in isolated rat CA and the possible mechanisms involving cyclic guanosine rnonophosphate （cGMP） dependent protein kinase （PKG）. The isolated endothelium-intact and endothelium-denuded rat CA rings were treated with HR injury. Evaluation of endothelium-dependent and -independent vasodilation relaxation of the CA tings were performed using wire myography and the protein expressions were assayed by Western blotting. SCU （10-1 000 μmol.L^-1） could relax the endothelium-intact CA rings but not endothelium-denuded ones. In the intact CA rings, the PKG inhibitor, Rp-8-Br-cGMPS （PKGI-rp, 4 μmol.L ^-1）, significantly blocked SCU （10-1 000 μmol.L^-1）-induced relaxation. The NO synthase （NOS） inhibitor, NO-nitro-L-arginine methylester （L-NAME, 100 μmol.L^-1）, did not significantly change the effects of SCU （10-1 000 μmol.L^-1）. HR treatment significantly impaired ACh-induced relaxation, which was reversed by pre-incubation with SCU （500 μmol.L^-1）, while HR treatment did not altered NTG-induced vasodilation. PKGI-rp （4 μmol.L^-1） blocked the protective effects of SCU in HR-treated CA tings. Additionally, HR treatment reduced phosphorylated vasodilator-stimulated phosphoprotein （p-VASP, phosphorylated product of PKG）, which was reversed by SCU pre-incubation, suggesting that SCU activated PKG phosphorylation against HR injury. SCU induces CA vasodilation in an endothelium-dependent manner to and repairs HR-induced impairment via activation of PKG signaling pathway.

关 键 词：SCUTELLARIN Endothelium-dependent vasodilation Hypoxia reoxygenation c GMP-dependent protein kinase RAT Coronary artery 

分 类 号：R965[医药卫生—药理学]