结直肠癌患者不同组织中K-ras基因研究  

Research of K-ras Gene of Colorectal Cancer at Different Tissue

作　　者：宋枫[1] 高峰[1] 吴伟强[1] 杨增强[1] 徐明[1] 张鑫[1] 

机构地区：[1]兰州军区兰州总医院肛肠外科,730050

出　　处：《医学研究杂志》2015年第3期75-77,共3页Journal of Medical Research

基　　金：甘肃省自然科学基金资助项目(1308RJZA154);兰州军区医药卫生科研计划基金资助项目(CLZ12JB15)

摘　　要：目的研究结直肠癌患者不同组织中K-ras基因的临床意义。方法采用实时荧光定量PCR方法检测患者原发灶组织100例、转移淋巴结组织64例、远处转移灶13例、腺瘤组织19例中K-ras基因突变情况。结果结直肠癌原发灶、转移淋巴结组织、远处转移灶、腺瘤组织的K-ras基因检出率分别为100%、92.2%、92.3%、89.5%;K-ras基因突变率分别为39.0%、30.5%、33.3%、17.6%。主要突变基因第12编码子34位G>T,G>A,G>C,35位G>A,G>T,G>C;第13编码子37位G>C,38位G>A等8种基因发生突变。结论结直肠癌原发灶、转移淋巴结组织、远处转移灶的K-ras基因突变率基本一致,而腺瘤组织K-ras基因突变率明显低于原发灶、转移淋巴结组织、远处转移灶组织。K-ras基因突变在结直肠癌的发生、发展中起重要作用,这对结直肠癌的防治有重要的指导意义。Objective The Research of K - ras gene of colorectal cancer at different tissue. Methods One-hundred primary foci, 64 metastatic lymph nodes, 13 metastatic remoter organs and 19 colorectal adenoma from colorectal cancer patients were collected Real - time fluorescence quantitative PCR were performed to detct K - ras mutations results were analyzed. Results The frequency of K - ras detct in primary foci, metastatic lymph nodes, metastatic remoter organs and colorectal adenoma were 100% ,92. 2% ,92. 3% and 89.5%. The frequency of K - ras mutation in primary foci, metastatic lymph nodes, metastatic remoter organs and colorectal adenoma were 39.0% ,30. 5% ,33.3% and 17.6%. The most common mutation of codon was 12 （G 〉 T,G 〉 A,G 〉 C,G 〉 A,G 〉 T,G 〉 C） and codon 13 （ G 〉 C,G 〉 A）. Conclusion The detection of K - ras mutations in primary focl, metastatic lymph nodes, metastatic remoter organs was concordant. K - ras mutations play an important role in the carcinogenesis and development of eolorectal cancer.

关 键 词：K-RAS基因 结直肠癌 基因突变 

分 类 号：R735[医药卫生—肿瘤]