香菇多糖联合顺铂对Lewis肺癌荷瘤小鼠肿瘤S-100蛋白表达影响的研究  

The combined effect of lentinan and DDP on the expression of S-100 protein and m RNA in Lewis lung carcinoma-bearing mice

作　　者：井欢[1] 于宁[2] 唐莹[1] 关洪全[1] 

机构地区：[1]辽宁中医药大学基础医学院病理教研室,辽宁沈阳110032 [2]辽宁中医药大学第一临床学院,辽宁沈阳110032

出　　处：《解剖科学进展》2015年第2期139-141,共3页Progress of Anatomical Sciences

基　　金：辽宁省教育厅资助项目(L2010355)

摘　　要：目的研究香菇多糖(lentinan,LNT)联合顺铂(DDP)对荷瘤小鼠实体瘤中S-100蛋白表达的影响。方法体外培养Lewis肺癌小鼠肿瘤细胞,接种健康BALB/c小鼠,荷瘤成功后予以治疗,实验分组为:荷瘤组,LNT组,DDP组以及LNT+DDP组。观察小鼠一般情况,并采用免疫组化法检测S-100蛋白表达,RTPCR检测S-100基因表达。结果肿瘤组织内S-100蛋白和m RNA表达水平LNT组、DDP组或LNT+DDP组比荷瘤组增多(<0.05),LNT+DDP组比LNT组和DDP组增多(<0.05)。结论 LNT同DDP联合使用上调S-100蛋白和基因的表达有协同作用。Objective To study the effects of lentinan（LNT） combined with cisplatin（DDP） on the expression of S-100 protein and m RNA in Lewis lung carcinoma-bearing mice. Methods Tumor cells of Lewis lung carcinoma were cultured in vitro and inoculated into healthy BALB/C mice. The mice were randomized into tumor-bearing group, LNT group, DDP group and LNT＋DDP group. The expression of S-100 protein and m RNA was detected by immunohistochemical and RT-PCR respectively. Results The expression levels of S-100 protein and m RNA were significantly higher in LNT group, DDP group or LNT＋DDP group than in tumor-bearing mice group（P〈 0.05）, lower in LNT group or DDP group than in LNT＋DDP group（P〈 0.05）. Conclusion LNT and DDP have synergistic effect for the upregulated expression of S-100 gene and protein in Lewis lung carcinoma.

关 键 词：肺癌 香菇多糖 顺铂 S-100 BALB/C小鼠 

分 类 号：R734.2[医药卫生—肿瘤]