青岛市秋季空气微生物群落多样性  

Phylogenetic diversity of airborne microbes in Qingdao downtown in autumn

作　　者：王琳[1] 宋志文[1] 徐爱玲[1] 吴等等 夏岩[1] 

机构地区：[1]青岛理工大学,山东青岛266033

出　　处：《应用生态学报》2015年第4期1121-1129,共9页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目(31170509)资助

摘　　要：采用KC-6120空气综合采样器采集空气微生物样品,通过构建16S/18S r DNA克隆文库方法分析青岛市市区街道秋季空气微生物群落结构特征.结果表明:空气细菌分布在6大类,分别为变形菌门(78.8%)、厚壁菌门(14.6%)、放线菌门(4.0%)、浮霉菌门(1.3%)、蓝藻门(0.7%)和栖热菌门(0.6%),优势菌属为不动杆菌属(39.7%)、葡萄球菌属(11.3%)、鞘脂单胞菌属(8.6%)和副球菌属(6.0%).空气真菌分布在子囊菌门(97.5%)和担子菌门(2.5%),优势菌属为核腔菌属(76.5%)、炭角菌属(13.6%)和外瓶霉属(2.5%).空气微生物中存在不动杆菌属、鞘脂单胞菌、葡萄球菌等致病菌或条件致病菌,以及引发多种农作物枯萎死亡的麦类核腔菌、团炭角菌和角状平脐疣孢等真菌.To determine the community structure of airborne microbes in Qingdao downtown in au- tumn, the airborne bacteria and fungi were collected by the KC-6120 air sampler and analyzed using the 16S/18S rDNA gene clone library method. Phylogenetic analysis of airborne bacteria showed that they belonged to six major phylogenetic groups： Proteobacteria （78. 8%）, Firmicutes （ 14.6%）, Actinobacteria （4.0%）, Planctomycetes （ 1.3%）, Cyanobacteria （0.7%）, and Deino- coccus-Thermus （0. 7%）. The dominant genera of airborne bacteria included Acinetobacter （39.7%）, Staphylococcus （11.3%）, Sphingomonas （8.6%）, Paracoccus （ 6.0% ） and Massilia （5.3%）. The main types of airborne fungi were Ascomycota （97.5%） and Basidiomycota （2.5%）. Dominant genera of airborne fungi included Pyrenophora （76.5%）, Xylaria （13.6%） and Exophiala （2.5%）. The pathogens or conditioned pathogens, such as Acinetobacter, Staphylococcus, or Sphin- gomonas were detected in the airborne bacteria, whereas certain kinds of fungi, such as P. graminea, X. hypoxylon and Zusmidium angulare that could cause a variety of crop diseases were also detected.

关 键 词：克隆文库 空气细菌 空气真菌 群落结构 微生物多样性 

分 类 号：X172[环境科学与工程—环境科学]