NDRG2对成年大鼠室管膜下区神经干细胞增殖的影响  

The influence of NDRG2 on the proliferation of neural stem cell in SVZ

作　　者：杨世畅 史明[1] 胡耿瑶[1] 吴中亮[1] 

机构地区：[1]第四军医大学西京医院神经内科,西安710032

出　　处：《神经解剖学杂志》2015年第2期237-241,共5页Chinese Journal of Neuroanatomy

基　　金：全军医学科技"十二五"科研重点项目(BWS11J013)

摘　　要：目的:探讨NDRG2(N-myc downstream regulated gene 2)对成年大鼠侧脑室室管膜下区(subventricular zone,SVZ)神经干细胞增殖的影响。方法:成年雄性Sprague-Dawley大鼠20只,随机分为对照组(n=10)、实验组(n=10)两组。实验组给予侧脑室注射过表达NDRG2的腺病毒,对照组给予侧脑室注射腺病毒空载体。采用Brd U免疫荧光染色法检测细胞增殖情况,Tunel染色检测细胞凋亡情况。结果:Brd U染色结果显示:实验组大鼠侧脑室室管膜下区Brd U阳性细胞显著少于对照组(P<0.05);Tunel染色提示:两组大鼠侧脑室室管膜下区凋亡的细胞数未见明显差异(P>0.05)。结论:上调NDRG2在成年大鼠脑内的表达,会抑制其侧脑室室管膜下区神经干细胞的增殖,但不影响其细胞的存活。Objective： To study the influence of NDRG2 on the proliferation of the neural stem cells in the subventricular zone（ SVZ）. Methods： Twenty male Sprague-Dawley rats were randomly divided into two groups,the control group（ n = 10） and the experimental group（ n = 10）. The rats in the experimental group were injected with adenovirus which overexpressed the NDRG2 gene in the lateral ventricle. The rats in the control group were injected with vehicle vector in the lateral ventricle. Brd U Immunofluorescent staining was used to test the cell proliferous status. Tunel staining was used to test the cell apoptotic status. Results： Brd U staining showed that the number of Brd U-positive cells in the SVZ was much fewer in the experimental group than that in the control group. The difference was statistical difference（ P 〈 0. 05）.Tunel staining showed that the number of apoptotic cells was comparable between the two groups,and there was no statistical difference between the two groups（ P 〉 0. 05）. Conclusion： Overexpression of NDRG2 in rat brain suppressed the proliferation of the neural stem cells in SVZ but did not affect cell survival.

关 键 词：NDRG2 神经发生 SVZ 大鼠 

分 类 号：R741[医药卫生—神经病学与精神病学]