纳米二氧化钛-氧化锌/硅溶胶/导电胶复合材料固定联吡啶钌制备磷酸可待因电化学发光传感器  

Preparation of Electrochemiluminescence Sensor by Immobilizing Tris (2,2'-bipyridine) Ruthenium (Ⅱ) on Gold Electrode with NanoTiO_2-ZnO/Silica Sol/Conductive Adhesive Composite Film

作　　者：杨兰兰[1] 李利军[1,2] 罗应[2] 张瑞瑞[1] 崔越[1] 崔福海[1] 孙科[1] 李彦青[1] 

机构地区：[1]广西科技大学生物与化学工程学院,柳州545006 [2]广西科技大学化学院,柳州545616

出　　处：《分析化学》2015年第4期547-552,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.31060219)资助~~

摘　　要：利用硅溶胶的成膜性、纳米二氧化钛-氧化锌大的比表面积及导电胶的粘结性,制备了纳米二氧化钛-氧化锌/硅溶胶/导电胶复合材料,基于此复合材料将联吡啶钌固定到金电极表面,制备了磷酸可待因电化学发光（ECL）传感器。在优化的实验条件（800 V负高压、扫描速度100 m V/s,磷酸盐缓冲体系（p H 6.5））下,可待因浓度在1.0×10^-7-1.0×10^-4mol/L范围内与电化学发光强度呈良好的线性关系（r^2=0.9973）,检出限为2.56×10^-8mol/L（S/N=3）。传感器表现出良好的重现性与稳定性,连续平行测定1.28×10^-5mol/L可待因溶液10次,发光强度的相对标准偏差（RSD）为2.7%;室温下保存10天后,发光强度为初始值的92%以上。测定可待因药物实际样品的加标回收率在99.3%-102.5%之间。Based on the film formability of silica sol,large specific surface area of Nano Ti O2-Zn O complex and adhesion of conductive adhesive（ CA）,Nano Ti O2-Zn O / Silica sol / CA composite material was prepared to immobilize Ru（ bpy）2 ＋3on the surface of gold electrode for the fabrication of electrochemiluminescence（ ECL）sensor of codeine phosphate. Under the optimal conditions such as 800 V negative high voltage,scan rate of100 m V / s and 0. 1 mol / L phosphate buffer（ p H 6. 5）,the ECL intensity showed a linear relationship with codeine concentration in the range of 1. 0 × 10^-7- 1. 0 × 10^-4mol / L（ R^2= 0. 9973） with the detection limit（ S /N = 3） of 2. 56 × 10^-8mol / L. The ECL sensor exhibited excellent repeatability and stability. The RSD of ECL intensity was 2. 7% for 1. 28 × 10^-5mol / L codeine under 10 continuous cyclic scans. The ECL response maintained over 92% of the original value after storage at room temperature for 10 days. The recovery was99. 3%- 102. 5% in the determination of real codeine phosphate sample.

关 键 词：电化学发光 联吡啶钌 硅溶胶 纳米二氧化钛/氧化锌 导电胶 可待因 

分 类 号：TP212.2[自动化与计算机技术—检测技术与自动化装置] O657.1[自动化与计算机技术—控制科学与工程]