HPLC法测定新复方大青叶片中对乙酰氨基酚、咖啡因、没食子酸的含量  

Determination of Content of Paracetamol、Caffeine、Gallic acid in New Compound Folium Isotidis by HPLC

作　　者：林晨[1] 

机构地区：[1]福建省药品检验所,福州350001

出　　处：《海峡药学》2015年第3期49-51,共3页Strait Pharmaceutical Journal

摘　　要：目的建立高效液相色谱法测定新复方大青叶片中对乙酰氨基酚、咖啡因、没食子酸的方法。方法采用Phenomenex Luan C18柱（250mm×4.6mm,5μm）色谱柱,以0.1%磷酸溶液-乙腈（80∶20）为流动相,流速为1.0mL·min^-1,检测波长为210nm。结果对乙酰氨基酚在38.02~380.25μg范围内,呈良好的线性关系（r=0.9998）,平均回收率为99.92%,RSD为0.28%（n=9）;咖啡因在3.838~38.375μg范围内,呈良好的线性关系（r=0.9999）,平均回收率为99.67%,RSD为0.18%（n=9）;没食子酸在1.08~10.8μg范围内,呈良好的线性关系,（r=0.9999）,平均回收率为98.34%,RSD为0.60%（n=9）。结论本方法准确、重复性好,可用于该制剂的质量控制。OBJECTIVE To establish A HPLC method for determination of the content of Paracetamol、Caffeine simultaneously and Gallic acid in New Compound Folium Isotidis.METHODS HPLC was used with Phenomenex Luria C18column（250 mm × 4.6mm,5μm）,acetonitrile-0.1% phosphoric acid（20∶ 80） as mobil phase and detection wavelength was 210 nm,flow rate was 1.0mL·min^-1.RESULTS The cabibration cures were liner in the range of38.02 ~ 380.05μg·mL^-1（r=0.9998） for Paracetamol; in the range of 3.838 ~ 38.375μg·mL^-1（r=0.9998） for Caffein; in the range of 1.08 ~ 10.8μg ·mL^-1（r=0.9998） for Gallic acid.The average recoveries were 99.8%,RSD=0.99%（n=6）; 99.8%,RSD=0.99%（n=6）; 99.8%,RSD=0.99%（n=6）; respectirely.CONCLUSION The method is simple,realizable and reproducible.It can be used effectiely for quality control of New Compound Folium Isatidis Tablets.

关 键 词：新复方大青叶片 对乙酰氨基酚 咖啡因 没食子酸 HPLC 

分 类 号：R927.2[医药卫生—药学]