外周血单个核细胞在Sonic hedgehog信号通路中的活化表达  

Activation of expression in peripheral blood mononuclear cells in Sonic hedgehog pathway

作　　者：赵乾焜 梁森[2] 闫慧明[2] 

机构地区：[1]山西医科大学,太原030001 [2]山西医科大学第二医院,太原030001

出　　处：《中华普通外科学文献（电子版）》2015年第2期12-14,共3页Chinese Archives of General Surgery(Electronic Edition)

基　　金：山西省卫生厅科技攻关项目(200920)

摘　　要：目的探讨Sonic hedgehog阻断抗体(Shh Ab)对外周血单个核细胞(PBMCs)抗胃癌MC细胞作用的表达。方法 Ficoll密度梯度离心法分离正常人PBMCs,并与胃癌MC细胞(GES-1细胞经亚硝酰胺类化合物处理)建立共培养体系;RT-PCR观察Shh、Gli-1基因的表达并进行半定量数据分析;于共培养体系中加入Shh阻断抗体,流式细胞术检测CD3、CD5、CD69分子表达。结果RT-PCR结果显示Gli-1m RNA在MC+Shh Ab细胞组值为0.284 5±0.002 5,低于MC细胞组的0.516 7±0.010 9(P<0.05);流式细胞检测Shh Ab可促进CD3、CD69分子表达,对CD5分子没有显著影响;Shh Ab增强PBMCs对MC细胞的杀伤。结论 Shh Ab可促进PBMCs化,增强PBMCs抗亚硝酰胺类化合物致癌机制的作用。Objective To discuss the expression of Sonic hedgehog blocking antibody （Shh Ab） in peripheral blood mononuclear cells（PBMCs） of anti-gastric cancer cells. Methods Healthy human PBM-Cs were centrifugated by Ficoll density gradient （GES-1 cells were treated by nitrite amides） and gastric cancer cells were co-cultured with MC. To observe the expression and semi-quantitative analysis of Shh and Gli-1 by RT-PCR, Shh blocking antibodies were added in the co-culture system, and the expression of CD3, CD5, CD69 was assayed by flow cytometry. Results RT-PCR showed that Gli-1mRNA in MC＋Shh Ab cell group was 0.284 5±0.002 5, lower than the MC cell group 0.516 7 ± 0.010 9 （P〈0.05）. Flow cytometry showed Shh blocking antibodies promoted the expression of CD3 and CD69. Shh Ab enhanced cell killing PBMCs for MC. Conclusion Shh Ab can promote activation of PBMCs, and enhances anti-carcinogenic effect of nitrite amides.

关 键 词：Sonic hedgehog蛋白 GES-1细胞 MNNG 淋巴细胞 

分 类 号：R735.2[医药卫生—肿瘤]