Effects of Mutagenesis by UV Irradiation and ^(60)Co-γ Irradiation on Fermentation of Xylose to Ethanol by Pichia stipitis  

紫外和^(60)Co-γ诱变对树干毕赤酵母发酵木糖产乙醇的影响（英文）

作　　者：刘阳[1] 曾璐[2] 熊冬梅[2] 熊兴耀[2,3] 苏小军[2] 蔡柳[2] 

机构地区：[1]湖南农业大学湖南省作物种质创新与资源利用重点实验室,湖南长沙410128 [2]湖南农业大学食品科技学院,湖南长沙410128 [3]中国农业科学院蔬菜花卉所,北京100081

出　　处：《Agricultural Science & Technology》2015年第3期426-430,446,共6页农业科学与技术（英文版）

基　　金：基金项目国家973计划课题(2012CB723004);湖南农业大学青年科学基金(12QN11)~~

摘　　要：ln this study, effects of UV irradiation and 60Co-γ irradiation on fermenta-tion of xylose to ethanol by Pichia stipitis were analyzed to investigate the optimal mutagenic conditions. According to the growth curve and fermentation curve of P. stipi-tis, the optimal incubation duration and fermentation duration of P. stipitis mutant strain were 18 and 48 h, respectively. The cel concentration of original yeast liquid was 107 cel s/ml. After mutagenesis by UV irradiation and 60Co-γ irradiation, yeast liquid was incubated in 20 g/L xylose media for 48 h. According to the results, after UV irradiation for 45-75 s, transformation efficiency reached 0.3794 g/g, which was 74.39% of the theoretical value; after irradiation with 800-1 000 Gy 60Co-γ, transforma-tion efficiency reached 0.3165 g/g, which was 62.06% of the theoretical value. Therefore, both UV irradiation and 60Co-γ irradiation could improve the efficiency of xylose fermentation to ethanol by P. stipitis under appropriate conditions.以树干毕赤酵母为出发菌株,研究紫外短波照射和60Co-γ辐照2种诱变方法对该菌种利用木糖产乙醇性能的影响,探讨较佳诱变条件。通过绘制菌体生长曲线及发酵曲线,确定了诱变菌最适宜的培养时长为18 h,诱变菌最适宜的发酵时长为48 h。原菌液浓度为107个/ml时,根据诱变后菌液在木糖浓度20 g/L中培养48h的结果可知,紫外处理45~75 s乙醇产率可达0.379 4 g/g,为理论值的74.39%,辐照处理800~1 000 Gy,乙醇产率可达0.316 5 g/g,为理论值的62.06%。因此,紫外及辐照这2种诱变方式在合适的参数条件下均能提高树干毕赤酵母利用木糖产乙醇的转化率。

关 键 词：Pichia stipitis MUTAGENESIS XYLOSE FERMENTATION ETHANOL 

分 类 号：TQ223.122[化学工程—有机化工] TQ926.4[轻工技术与工程—发酵工程]