高效液相色谱法检测猪肉中盐酸克伦特罗的前处理方法  

Research on pre-treatment method for HPLC determination of clenbuterol hydrochloride in pork

作　　者：朱晓艾[1] 陈雅君[1] 陈雪香[1] 贺丽苹[1,2] 夏旭[1] 曹庸[1] 

机构地区：[1]华南农业大学食品学院,广东广州510642 [2]华南农业大学测试中心,广东广州510642

出　　处：《食品与机械》2015年第2期106-109,共4页Food and Machinery

基　　金：国家科技支撑计划课题(编号:2012BAK17B00)

摘　　要：通过优化样品前处理方法和高效液相的检测条件,建立快速简便测定猪肉组织中盐酸克伦特罗的高效液相色谱法。结果表明,优化后的样品前处理方法只需提取、蛋白质沉淀两个过程,且采用C18色谱柱,流动相乙腈∶0.02 mol/L乙酸铵溶液（含0.1%甲酸）=25∶75,流速1.0 m L/min,进样量20μL,检测波长243 nm,可以获得良好的色谱峰,检测限为0.025 mg/kg,样品加标回收率达88.55%-94.03%,精密度RDS〈2%。该方法能达到准确、快速、低成本、简便的要求,适用于猪肉组织中盐酸克伦特罗的快速检测。The method of detection of clenbuterol hydrochloride in pork by high-performance liquid chromatography was established after simplifying the steps of the national standard method to optimize the chromatographic conditions..The results showed that,the optimized conditions just require two processes of extraction and protein precipitation,by using Alsonsil C18 HPLC column,optimized parameters for HPLC running were mobile phase ratio of acetonitrile to ammonium acetate 25 ∶ 75,flow rate1.0 m L / min,injection volume 20 μL,detection wavelength 243 nm and detection limit was 0.025 mg / kg.The coefficient of recovery was up to88.55% - 94.03% and the relative standard deviation（RSD） was less than 2%.This method is accurate,rapid,low-cost and simple,which is suitable for rapid detection of clenbuterol in pork.

关 键 词：盐酸克伦特罗 猪肉组织 高效液相色谱法 样品前处理 

分 类 号：S859.84[农业科学—临床兽医学]