Characterization of New Allelic Variation for Glutenin in EMS-Mutant Durum Wheat Population （Triticum turgidum L. subsp, durum （Desf.））  

作　　者：Elyadini Meryem Labhilili Mustapha Bentata Fatiha Azeqour Mohamed Taghouti Mouna Kahama Issa Martin Perry Gaboune Fatima Nasserlah Nessrelah Nachit Miloud 

机构地区：[1]Biotechnology Research Unit, Rabat Regional Agronomic Research Center, INRA, Avenue Mohamed Belarhi Alaoui, B. P. 6356Rabat-lnstitute, Rabat 10101, Morocco [2]Faculty of Sciences and Technology, Universite Hassan H Mohammedia, B. P. 146, Mohammedia 20800, Morocco [3]Faculty of Sciences and Technology, Semlalia, B. P. 2390, Marrakech-Maroc 40000, Morocco [4]Rothamsted Research Institute, Harpenden, Herts AL5 2JQ, UK [5]Settat Regional Agronomic Research Center, INRA, B. P. 589, Settat 26000, Morocco [6]ICARDA, P. O. Box 6299, Rabat-lnstitut, Rabat 10101, Morocco

出　　处：《Journal of Life Sciences》2014年第11期880-888,共9页生命科学（英文版）

摘　　要：The High Molecular Weight HMW-GS and Low-Molecular-Weight LMW-GS Glutenin Subunits are major determinants of wheat dough processing qualities. Tilling populations was generated by EMS mutagenesis from Chaml durum variety. Protein quality was investigated by SDS sedimentation, protein content, gluten content and thousand kernel weights. 21 mutants were selected from 1,500 lines of tilling population depending on the variations of their electrophoresis profiles. The analysis of Glu-B 1 HMW-GS has showed two types of profiles： lines deficient on Bx7 and lines with expression of new protein between （Bx7-ByS） allelic pair combination. The majority of these mutant lines have showed quality compounds associated with bad technological characteristics like the parent Cham l with the exception of two mutant lines expressing new protein that have significantly stronger gluten properties an one mutant deficient on Bx7 HMW-GS that developed high value of SDS. For Glu-B3 LMW-GS coding for LMW glutenin type 1 and 2, new mutant line showing expression of new protein pattern. The mutant showing over-expression of bands on gel SDS-PAGE for LMW typel like Chaml, have produced the highest protein and gluten content, while the new mutant showing new profile have showed high value of Gluten and SDS.

关 键 词：HMW-GS LMW-GS Triticum durum tilling population. 

分 类 号：S512.1[农业科学—作物学] TQ924[轻工技术与工程—发酵工程]