多黏芽孢杆菌HD-1产β-甘露聚糖酶的条件优化  

Optimizing Conditions of Producing the β-mannanase from Bacillus polymyxa HD-1

作　　者：程爱芳[1] 邓政东[1] 黄芳一[1] 陈文[1] 周念波[1] 

机构地区：[1]武汉生物工程学院生命科学与技术学院,武汉430415

出　　处：《湖北农业科学》2015年第1期164-167,共4页Hubei Agricultural Sciences

基　　金：武汉生物工程学院校级研究项目(2013JYI01)

摘　　要：采用DNS比色法,通过单因素试验和正交试验对多黏芽孢杆菌(Bacillus polymyxa)HD-1产β-甘露聚糖酶的培养基主要成分和培养条件进行优化。结果表明,该菌种产β-甘露聚糖酶的最适培养基为魔芋粉0.75%、酵母膏1.00%、磷酸二氢钾0.04%、硫酸镁0.05%,培养基初始p H 9.0;最适培养条件为接种量6%,培养温度31℃,培养时间27 h。在此优化条件下,多黏芽孢杆菌产β-甘露聚糖酶活性可达72.6U/m L。The colorimetric method of DNS, single factor and orthogonal experiments were used to optimize the fermentation medium and culture conditions of Bacillus polymyxa HD-1 to improve the production of β-mannanase. The results showed that the optimal medium contained 0.75% konjac,1.00% yeast extract,0.04% KH2PO4·3H2O, 0.05% Mg SO4·7H2O, with the initial p H9.0. The optimal culture conditions were 6% inoculation, culture temperature of 31 ℃, and culturing length 27 of hours. Under these conditions, the β-mannanase produced from Bacillus polymyxa HD-1 was 72.6 U/m L.

关 键 词：多黏芽孢杆菌(Bacillus polymyxa) Β-甘露聚糖酶 发酵条件 优化 

分 类 号：Q939.97[生物学—微生物学]