沙棘枝叶与浆果黄酮抗氧化活性比较  

Antioxidant Activity of Flavonoids from Branches-Leaves and Berry of Hippophae rhamnoides Linn

作　　者：焦扬[1] 李彩霞[1] 高海宁[1] 冯九海 

机构地区：[1]河西学院农业与生物技术学院 [2]甘肃省应用真菌工程实验室,张掖734000

出　　处：《天然产物研究与开发》2015年第4期655-660,共6页Natural Product Research and Development

基　　金：国家中医药管理局2012年中医药行业科研专项(201207002);甘肃省高校河西走廊特色资源利用省级重点实验室科研资助项目(XZ12-2)

摘　　要：以大果沙棘浆果黄酮(ZYCFJ)为参照,考察了张掖大果沙棘黄酮(ZYCF)和新疆野生沙棘枝叶黄酮(XJCF)的抗氧化活性。结果表明:沙棘浆果黄酮和沙棘枝叶黄酮对不同自由基的清除效果均有差异。在黄酮浓度为50μg/m L时,ZYCFJ、ZYCF和XJCF对ABTS+清除率分别为60.8%、42.6%、45.4%;对DPPH-的清除率分别为95.7%、80.6%、88.6%,在黄酮浓度高于40μg/m L时,ZYCFJ对DPPH-和ABTS+的清除效果比ZYCF和XJCF的好。在黄酮浓度为500μg/m L时,ZYCFJ、ZYCF和XJCF对·OH的清除率分别为92.7%、88.9%、98.6%;对O-·2的清除率分别为76.5%、70.6%、82.4%;对亚硝酸盐的清除率分别为98.7%、76.8%、83.7%。就对亚硝酸盐的抑制和Fe3+的还原来说,ZYCFJ明显优于ZYCF和XJCF;对超氧阴离子和羟自由基的清除效果ZYCFJ、ZYCF和XJCF的相当。Uslng flavonoids from Hippophae rharnrtoides L. berry （ZYCFJ） as reference,the antioxidant activities of fla- vonoids in branches and leaves of Hippophae rhanmoldes L. from Zhangye （ZYCF） and Xinjiang （XJCF） were evaluated by measuring their scavenging activity against DPPH,ABTS,hydroxyl,superoxide anion radicals and reducing Fe^3 ＋ and inhibiting nitroso. The results showed that at 50 μg/mL concentration level, the clearance rates of ZYCFJ, ZYCF and XJCF on ABTS^ ＋ were 60.8% ,42.6% ,45.4%, and on DPPH＇were 95.7% ,80.6%. 88.6%. When flavonoids concentrations higher than 40 μg/mL, the scavenging activity against DPPH and ABTS ^＋ of ZYCFJ was better than XJCF and ZYCF. At 500 μg/mL concentration level,the scaveng/ng rates of ZYCFJ,ZYCF and XJCF on · OH were 92.7%, 88. 9% ,98.6% ,and on O^2-were 76.5% ,70.6% ,82.4% ,and inhibition ratio of ZYCFJ, ZYCF and XJCF on nitrite were 98.7% ,76.8% ,83.7%. In term of the inhibition rates of nitrite and the reduction of Fe^3＋ ,ZYCFJ was better than that of ZYCF and XJCF. The scavenging effect of ZYCFJ,ZYCF and XJCF on superoxide anion and hydroxyl free radical were equivalent.

关 键 词：沙棘枝叶 黄酮 张掖大果沙棘 新疆野生沙棘 抗氧化性 

分 类 号：R285[医药卫生—中药学]