新鲜蛹虫草中SOD的提取工艺优化  

Optimization on Extraction Process of SOD from Fresh Cordyceps Militaris

作　　者：李聪[1] 龚利媚 张兆霞[1] 李泳[1] 

机构地区：[1]广东海洋大学理学院,广东湛江524088

出　　处：《化学与生物工程》2015年第4期48-50,共3页Chemistry & Bioengineering

基　　金：广东海洋大学大学生创新实验项目(1056613084)

摘　　要：以酶活力为考核指标,对新鲜蛹虫草中超氧化物歧化酶(SOD)的提取工艺进行了优化,通过单因素实验和L9(34)正交实验确定新鲜蛹虫草中SOD的最优提取条件为:新鲜蛹虫草用量0.5g、磷酸缓冲溶液用量20mL、磷酸缓冲溶液pH值8.0、超声提取时间20min、(NH4)2SO4饱和度42%,在此条件下,SOD酶活力最高。Using enzyme activity as evaluation index, the extraction process of SOD from fresh Cordyceps rnilitaris was optimized. Through single factor experiment and Lg （34） orthogonal experiment, the optimum ex- traction conditions were obtained as follows：amount of fresh Cordyceps rnilitaris was 0. 5 g, dosage of phos- phate buffer solution was 20 mL, pH value of phosphate buffer solution was 8.0,ultrasonic extraction time was 20 min, （NH4）2SO4 saturation was 42%. Under above conditions,the enzyme activity of SOD was the highest.

关 键 词：蛹虫草 SOD 酶活力 提取 

分 类 号：TQ461[化学工程—制药化工]