苎麻Genomic-SSR与EST-SSR分子标记遗传差异性分析  

Genetic Differences in Ramie Analyzed between Genomic-SSR and EST-SSR

作　　者：刘晨晨[1] 栾明宝[1] 陈建华[1] 王晓飞[1] 许英[1] 孙志民[1] 

机构地区：[1]中国农业科学院麻类研究所,长沙410205

出　　处：《中国麻业科学》2015年第2期57-63,共7页Plant Fiber Sciences in China

基　　金：国家自然科学基金(31471547;30900913);湖南省自然科学基金(13JJ4116);国家科技支撑计划(2013BAD01B03-13)

摘　　要：为了明确苎麻不同SSR的遗传差异性,利用Genomic-SSR与EST-SSR两种SSR标记对19个苎麻核心种质的遗传多样性进行分析。根据统计结果,Genomic-SSR平均检测到的观测等位基因数为3.4643、有效等位基因数为2.1982、Shannon多样性指数为0.8864、观测杂合度为0.5254、期望杂合度为0.5172;EST-SSR平均检测到的观测等位基因数为2.3333、有效等位基因数为1.9438、Shannon多样性指数为0.7120、观测杂合度为0.5128、期望杂合度为0.4778。Genomic-SSR和EST-SSR两种引物的有效等位基因数(Ne)和观测杂合度(Ho)差异不显著,Genomic-SSR的Shannon指数显著高于EST-SSR。研究结果表明,在标记多态性及基因型鉴别等方面,Genomic-SSR与EST-SSR均具有显著的遗传差异性,但是两种标记都能有效的鉴别不同基因型个体。Genomic-SSR可优先用于分子身份证、高密度遗传连锁图谱的构建和遗传多样性分析;EST-SSR用来研究苎麻近缘种间的遗传多样性、进化分析、连锁图谱和比较基因组学更有优越性。In order to clarify the genetic differences among different SSRs,Genomic -SSR and EST-SSR were used to analyze the genetic diversity of 19 important ramie genetic resources.According to the statistical results,the average observed -alleles detected by Genomic -SSR was 3.4643,effective alleles was 2.1982,shannon was 0.8864,heterozygosity was 0.5254,expected heterozygosity was 0.5172.The average observed -alleles detected by EST -SSR was 2.3333,effective alleles was 1.9438,shannon was 0.7120,heterozygosity was 0.5128,expected heterozygosity was 0.4778.There were no significant differences in effective number of alleles （Ne）and observed heterozygosity （Ho）be-tween Genomic -SSR and EST -SSR.Genomic -SSR Shannon index was significantly higher than that of EST -SSR.The above results showed there were significant genetic differences in marker polymor-phism and genotype identification between Genomic -SSR and EST -SSR,but the two kinds of markers could also effectively identify different genotypes.Genomic -SSR had priority for molecular identifica-tion,construction of high -density genetic linkage map and analysis of genetic diversity,EST -SSR had more advantages of studying the genetic diversity among close ramie species,evolutionary analysis,ge-netic map and comparative genomics.

关 键 词：苎麻 遗传差异性 分子标记 GENOMIC-SSR EST-SSR 

分 类 号：S563.1[农业科学—作物学]