Polylysine-modified Polyethylenimines as siRNA Carriers for Effective Tumor Treatment  

作　　者：Jie Chen Zi-xue Jiao Lin Lin Zhao-pei Guo Cai-na Xu Yan-hui Li 田华雨 Xue-si Chen 

机构地区：[1]Key Laboratory of Polymer Ecomaterials,Changchun Institute of Applied Chemistry,Chinese Academy of Science [2]School of Materials Science and Technology,Changchun University of Science and Technology

出　　处：《Chinese Journal of Polymer Science》2015年第6期830-837,共8页高分子科学（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(Nos.51222307,51303173,51390480,21474104 and 51403205);the Ministry of Science and Technology of China(International cooperation and communication program 2011DFR51090);Jilin Province Science and Technology Development Program(Nos.20120306,20130521011JH)

摘　　要：Polyethylenimine-poly（L-lysine） （PEI-PLL） copolymer was synthesized via ring-opening polymerization of L-lysine N-carboxyanhydride （Lys（Z）-NCA） initiated by PEI. The complexation of PEI-PLL with siRNA was studied by particle size and zeta potential measurements. The flow cytometric analysis and confocal imaging showed its excellent intracellular trafficking ability. PEI-PLL displayed higher gene silencing efficiency and lower cytotoxicity than commercial PEI-25k in vitro. In the antitumor study, PEI-PLL was further combined with siVEGF and showed obviously tumor inhibition effect for the treatment of CT26 tumor model. Therefore, PEI-PLL is a promising siRNA carrier candidate for further antitumor treatment in vivo.Polyethylenimine-poly（L-lysine） （PEI-PLL） copolymer was synthesized via ring-opening polymerization of L-lysine N-carboxyanhydride （Lys（Z）-NCA） initiated by PEI. The complexation of PEI-PLL with siRNA was studied by particle size and zeta potential measurements. The flow cytometric analysis and confocal imaging showed its excellent intracellular trafficking ability. PEI-PLL displayed higher gene silencing efficiency and lower cytotoxicity than commercial PEI-25k in vitro. In the antitumor study, PEI-PLL was further combined with siVEGF and showed obviously tumor inhibition effect for the treatment of CT26 tumor model. Therefore, PEI-PLL is a promising siRNA carrier candidate for further antitumor treatment in vivo.

关 键 词：Gene carrier Gene silencing POLYLYSINE Tumor treatment VEGF siRNA. 

分 类 号：R730.5[医药卫生—肿瘤]