MicroRNA-182在非小细胞肺癌中的表达及临床意义  

Clinical significance of miRNA-182 expression in non-small cell lung cancer

作　　者：陈晓林[1] 欧阳小平[1] 黄玉民[1] 陶玉坚[1] 

机构地区：[1]扬州市第一人民医院呼吸内科,江苏扬州225001

出　　处：《临床肺科杂志》2015年第6期997-1000,共4页Journal of Clinical Pulmonary Medicine

摘　　要：目的探讨微小RNA-182(miRNA-182)在非小细胞肺癌中的表达情况,并进一步分析其与肿瘤的临床病理特征和预后的相关性。方法收集40例非小细胞肺癌肿瘤组织、癌旁组织和正常组织,采用RT-PCR方法检测miRNA-182在组织中的相对表达量,并分析其与临床病理资料和预后的相关性。结果肿瘤组织中miR-182的表达水平明显高于正常肺组织和癌旁组织(P<0.05);miR-182的表达水平与临床分期、淋巴结转移和EGFR基因突变明显相关(P<0.05)。Kaplan-Meier分析显示肿瘤组织中miR-182低表达者生存时间明显低于高表达组,差异有显著统计学意义(P<0.05)。结论 miR-182在非小细胞肺癌中表达均上调,可作为肺癌转移和预后判断的一个新的标志物。Objective To investigate the expression of miRNA-182 in patients with non-small cell lung cancer （ NSCLC） and to analyze the association of the expression and prognosis. Methods The expression levels of miRNA-182 in 40 tumor tissues, paracanerous tissues and paired normal tissues of NSCLC were detected by using RT-PCR. The correlation between the level of miRNA-182 and the pathological characteristics and prognosis were also analyzed. Results The expression level of miRNA-182 was up-regulated in tumor tissues while compared with paracanerous tissues or paired normal tissues （P〈0. 05）. The expression of miRNA-182 was associated with genetic mutation of EGFR, lymphatic metastasis, and TNM stages （ P〈0. 05 ） . The expression of miRNA-182 was correlated with overall survival of NSCLC （P〈0. 05）. Conclusion The expression of miRNA-182 is up-regulated in tumor, and it maybe a single evaluation for predicting the prognosis of of NSCLC.

关 键 词：非小细胞肺癌 微小RNA-182 预后 EGFR 

分 类 号：R734.2[医药卫生—肿瘤]