高效液相色谱法测定酵母浸粉中维生素B_6的含量  

Determination of Content of Vitamin B_6 in Yeast Extracting Powder by HPLC

作　　者：刘跃芹[1] 吴延东[1] 赵雪松[1] 吴杰[1] 刘艳波[1] 闫寒[1] 

机构地区：[1]玉米深加工国家工程研究中心,长春130033

出　　处：《食品工业》2015年第4期258-260,共3页The Food Industry

基　　金：农业科技成果转化资金项目(2012GB2B100101)

摘　　要：建立了高效液相色谱法测定酵母浸粉中维生素B6含量的方法。色谱条件为Symmetry-C18柱（3.9 mm×150mm,5μm）;流动相：A相为甲醇,B相为含0.05 g/L的EDTA和1.1 g/L的庚烷磺酸钠的2.5%的乙酸溶液（p H 3.40）,以V（A）∶V（B）为1∶6比例等度洗脱;流速为0.8 m L/min;柱温为30℃;检测波长为280 nm;样品分析时间为10 min。结果表明：维生素B6的线性范围为0.10~100.00μg/m L（r=0.999 9）。该方法检出限位16.0μg/100 g,定量限为50.0μg/100 g。日间和日内精密度均小于15%,低、中、高三种浓度下酵母浸粉样品中维生素B6的提取回收率分别平均为93.16%±2.00%、96.70%±2.08%、93.76%±1.79%（n=4）。此法操作简便、快速、准确,可作为酵母浸粉中维生素B6含量的检测方法。The purpose was to establish a method for determining the content of vitamin 136 in yeast extracting powder using HPLC. The detection conditions were： using Symmetry-C18 column （3.9 mm×150 mm, 5 μm）, isocratic elution （V（A） ： V（B）=1 ： 6） and mobile phase A： methanol, phase B： 2.5% acetic acid solution containing 0.05 g/L EDTA and 1.l g/L sodium heptanesulfonate （pH 3.40）, flow rate： 0.8 mL/min, detection wavelength： 280 nm, sample analysis time was 10 rain. The linear ranges of vitamin B6 were 0.10-100 μg/mL （r=0.999 9）, The liunt of detection was 16.0 lag/100 g, and the liunt of quantification was 50.0 pg/100 g. relative standard deviation was less than 15%, recovery of the three concentration of vitamin B6 were 93.16%±2.00% , 96.70% ±2.08% and 93.76%±1.79% （n=4）. The method was simple, rapid and reliable. It can be used in determination of vilamin 136 in yeast extracting powder.

关 键 词：维生素B6 高效液相色谱法 酵母浸粉 

分 类 号：O657.72[理学—分析化学] TQ920.1[理学—化学]