中国荷斯坦牛乳腺中β-酪蛋白的变化研究  

Study on the change of β-casein in Chinese holstein mammary gland

作　　者：甄贞[1,2] 曲波[1,2] 姜毓君[3] 李庆章[1,2] 

机构地区：[1]东北农业大学生命学院,哈尔滨150030 [2]东北农业大学农业生物功能基因重点实验室,哈尔滨150030 [3]东北农业大学食品学院,哈尔滨150030

出　　处：《中国乳品工业》2015年第4期4-6,9,共4页China Dairy Industry

基　　金：国家自然科学基金(31100959)

摘　　要：对乳腺中β-酪蛋白在蛋白水平和基因水平的表达研究进行对比,系统阐述中国荷斯坦牛乳腺中β-酪蛋白的变化规律。结果表明:中国荷斯坦牛乳腺发育过程中,β-酪蛋白出现在妊娠中期,表达水平逐渐升高,至泌乳期达到最高,退化期逐渐下降。β-酪蛋白基因表达水平在青春期很低,到妊娠期迅速升高。整个泌乳期β-酪蛋白表达水平比较平稳,各时点无差异,而其基因表达水平却有显著差异。退化早期,β-酪蛋白及其基因表达水平开始逐渐下降,但仍与青春期有显著差异。In the study, theβ-casein of Chinese Holstein dairy cow mammary gland on the protein level and gene level were compared and the changing regular ofβ-casein were systematically expounded.The results showed thatβ-casein appeared mid-pregnancy during mammary growing, advanced gradually and reached the peak in lactation, and decreased step by step in involution. The expression ofβ-casein gene was very low in puberty, increased fast after early pregnancy. The level ofβ-casein was stable during lactation, and there was no difference among different time, however, the expression ofβ-casein gene had significant difference. The expression ofβ-casein and its gene beginned to descend gradually in early involution, however which had significant difference than that of puberty.

关 键 词：中国荷斯坦牛 乳腺 激光扫描共聚焦显微技术 Β-酪蛋白 

分 类 号：Q93-33[生物学—微生物学]